De faciltieit ontwikkelt en gebruikt, high-throughputmethoden toe voor genomisch onderzoek.

Increasing the efficiency of gene targeting (GT) as a genome editing tool in plants has been an important goal in plant biotechnology.

Remus Dame

In this thesis I highlight the applications of Oxford Nanopore Technologies (ONT) sequencing. This technique is a relatively new approach in the sequencing field, where nanopores are embedded in a membrane, DNA molecules are pulled through nanopores and an electrical current serving as the sequencing…

Parkinson's disease is the second most common neurodegenerative disease in the world. One of its symptoms is the loss of dopaminergic neurons in the substantia nigra pars compacta.

Promotor: P.J.J. Hooykaas, Co-Promotor: E.J. van der Zaal

The four possible segments A, T, C and G that link together to form DNA molecules, and with their ordering encode genetic information, are not only different in name, but also in their physical and chemical properties.

Every organisms in the tree of life faces the same challenge: the length of its DNA exceeds the volume of the cell it needs to fit in. Several strategies have evolved to solve this problem, one of them being the expression of proteins that bind and organize the DNA.

All life on earth contains DNA, which is used to store biological information. Organisms compact their DNA in order for it to fit inside their cell(s).

Climate change is a challenge for both current and future generations. New biological resources have to be developed in order to meet the demand for energy as well as the demand for food.

Promotie

This thesis focuses on two processes involved in fighting infections: metabolism and immune cell motility and navigation.

The increasing resistance of pathogenic bacteria to antibiotics necessitates the discovery of new medicines.

Bacterial biocatalysis constitutes a sustainable alternative for high-value chemicals production by enabling the utilization of renewable feedstocks.

This thesis pioneers diatom molecular identification and quantification through genome-scale methods, with four key aims: (i) reviewing DNA/RNA sequencing methods in aquatic biomonitoring to highlight their strengths and limitations;

The RNA guided endonuclease based on the CRISPR/Cas system of Streptococcus pyogenus is a potent new tool for genome engineering in plants.

The ongoing increase in antimicrobial resistance combined with the low discovery of novel antibiotics is a serious threat to our health care.

Fungal food spoilage often starts with a contamination with spores. Experimental data strongly indicate the existence of subpopulations of spores with different levels of resistance to preservation methods.

The human body consists of hundreds, perhaps thousands of different types of cells, each with different morphologies and functions, despite having the same genome.

Promotor: Jaap Brouwer, Co-promotor: Remus Th. Dame

Promotor: P. J. J. Hooykaas Co-promotor: B. S de Pater

Bacteria often experience external challenges, such as changes in environmental conditions or attacks by bacteriophages.

Promotie

In this thesis, I investigated the neurogenetic properties of Neotropical bat species. Bats represent the second largest mammalian order (Chiroptera) in the world, with Neotropical bats constituting about 13% of the total number of bat species.

Promotor: M. Ubbink, Co-Promotor: R. T. Dame

Structural variants (SVs) are the hidden architecture of the human genome, and are critical for us to understand diseases, evolution, and so on.

Biological polymers, including proteins and the genome, undergo folding processes crucial for their proper functioning. Even slight changes in the folding structure of these biopolymers can have significant implications, leading to the development of various pathological conditions, such as neurodegenerative…

Promotie

Promotie

Meer informatie over de organisatie van het CML is beschikbaar op de Engelse versie van de CML-website

Informatie over de organisatie van het Leiden Institute of Advanced Computer Science (LIACS) is beschikbaar in het Engels.

Hybrid zones occur where two species meet and produce offspring (hybrids). Typically, hybrids show a considerable reduction in fitness. In this thesis two hybrid zones are treated.

All crested and marbled newts (the genus Triturus) suffer from an unusual genetic abnormality, called a balanced lethal system, that kills exactly half of their offspring. How can a trait so disadvantageous have survived millions of years of natural selection?

The objective of this study was to investigate the expression and function of GRHL2 in different breast cancer subtypes.

Acidobacteria is a widely distributed phylum but their functional roles in ecosystem processes are still largely elusive.

Several single-stranded RNA viruses make use of Xrn1-resistant RNAs in their 3’ untranslated regions of their genome RNAs in order to increase their pathogenicity.

Supervisor: P.J.J. Hooykaas

This dissertation is part of a broader research project that revolves around unraveling the evolution of balanced lethal systems.

The haplolepideous mosses (Dicranidae) comprise about 4000 species distributed over a wide range of habitats, with great gametophytic and sporophytic morphological variation. Their monophyly is well supported by the results of several molecular phylogenetic studies, which shed light on their relationships…

The investigations described in this thesis lay out strategies aimed at advancing antibiotic research and development. The examples presented revolve around two main approaches: understanding drug-target interactions and target identification.

The AT-Hook Nuclear Localized 15 (AHL15) gene belongs to a plant-specific gene family of DNA-binding proteins. Overexpression of AHL15 in Arabidopsis thaliana delays developmental phase transitions whereas its loss of function causes a slight acceleration.

Understanding DNA repair mechanisms is essential for advancing plant biotechnology and crop improvement. This study investigates how double-strand breaks (DSBs), induced by CRISPR/Cas systems, are repaired in the model plant Arabidopsis thaliana, and how these processes influence Agrobacterium tumefaciens…

De organisatie van het Instituut Psychologie is in handen van het instituutsbestuur en het opleidingsbestuur. Diverse commissies leveren gespecialiseerde bijdragen, zoals de Commissie Ethiek en de Wetenschapscommissie. Studenten zijn vertegenwoordigd in opleidingscommissies.

De sectie Cognitieve Psychologie is als volgt georganiseerd:

De sectie Klinische Psychologie is als volgt georganiseerd:

Centrum van de Universiteit Leiden voor eerstegraads lerarenopleiding, nascholing docenten voortgezet onderwijs en professionalisering docenten hoger onderwijs.

Instituut Politieke Wetenschap