Promotor: Prof.dr. H.S. Overkleeft

Lysosomal acid glucosylceramidase (GBA1) is a lysosomal enzyme that degrades glucolipids with its main substrate being glucosylceramide (GlcCer). Defects in the GBA1 gene lead to glycosphingolipidosis Gaucher disease (GD), in which the hydrolysis of GlcCer is impaired and therefore, it accumulates in…

In the era of big data, extracting insights from complex datasets is a key challenge. This thesis demonstrates the superiority of graph-based methods over traditional clustering (e.g., k-means, DBSCAN) and outlier detection for analyzing high-dimensional and noisy data.

This thesis describes the use of an activity-based proteomics method to study the endocannabinoid system.

Science Based Business (SBB) verbindt de domeinen wetenschap, bedrijfsleven en beleid. Wetenschappelijk onderzoek waar we ons in Leiden op richten, bijvoorbeeld op het gebied van kunstmatige intelligentie, belooft onze samenlevingen ten goede te komen. Het vertalen van wetenschap naar positieve maatschappelijke…

Supramolecular polymers are class of materials that are formed by non-covalent interactions such as hydrogen bonding, π-π interactions, electrostatic interactions and the hydrophobic effect.

Promotor: H.S. Overkleeft, Co-promotor: M. van der Stelt

Promotores: H.S. Overkleeft, G.A. van der Marel, Co-promotor: B.I. Florea

Neuraminidases are enzymes that cleave glycosidic linkages of sialic acid. These enzymes are involved in influenza infections as well as in many cellular processes in mammals and micro-organisms.

Glycosidases are important enzymes in the turnover of polysaccharides and glycoconjugates, and are involved in a range of human pathologies including genetic disorders such as Gaucher and Pompe disease, but also in various cancers.

Promotor: K.J. Batenburg

In the last decades, activity-based protein profiling (ABPP) has emerged as a powerful chemical tool that may aid the ever-challenging drug discovery process.

Promotores: Prof.dr. W.J.G.M. Peijnenburg & Dr. M.G. Vijver

Microscopic insight on lipid-based nanomedicine in vivo remains limited to the perception of the knowledge that could be obtained: if we interpret only what we see, then we only believe to know. Although believing to know encapsulates an undefined amount of uncertainty in the exploration of lipid-based…

The main goal of this thesis is to achieve advanced control of binding properties in bis-pyrrolic and macrocyclic pyrrolic receptors by light.

Promotor: Prof.dr. H.A.G. Wijshoff

Glycosidases are essential in fundamental biological processes and are responsible for the degradation of most (oligo)saccharides, glycolipids and glycoproteins.

Lysosomal glycosidases are acid hydrolases that fragment glycoconjugates in lysosomes.

Promotor: H.S. Overkleeft, J. Neefjes, Co-promotor: M. van der Stelt

Promotores: Prof.dr.G.R. de Snoo and Dr. R. Heijungs

Business Process Model and Notation (BPMN) has become the standard for business processes diagrams.

The aim of this thesis was to develop aluminium salt-based nanoparticles that may ultimately be used as adjuvant in human vaccines.

Promotores: Wim Jiskoot; Ferry Ossendorp

Promotores: Prof.dr. H.S. Overkleeft, Prof.dr. G.A. van der Marel

This thesis describes the design, characterization and application of dextran based crosslinked network.

Promotor: Prof.dr. M. Danhof

The work described in this thesis focuses on the development of linear or cyclized peptide probes against protein N-methyltransferases to characterize their specific binding behavior, providing further binding details for inhibitory activity study.

Promotor: Prof.dr. E. Bouwman, Co-Promotor: S. Bonnet

In dit proefschrift wordt de samenwerking tussen studenten en resultaten in online projectgestuurd hoger onderwijs in China onderzocht.

Over the last decades, aviation safety has improved strongly. As a downside, airline pilots do not have as many opportunities to develop through experience the competencies that they need in critical situations.

This thesis explores squaramide-based supramolecular polymers and hydrogels, enhancing their mechanical strength for 3D cell culture.

A new type of tripodal squaramide-based supramolecular hydrogels is developed and studied.

Tin electrodeposition applications have rapidly evolved in the past 25 years.

Promotor: Prof.dr. J. Kuiper

Promotor: T. Hankemeier, Co-promotor: T.H. Reijmers

Inspired by natural photosynthesis, perovskite-based photoelectrochemical (PEC) systems are being developed for artificial photosynthesis, aiming to enhance solar-to-hydrogen conversion for green energy.

The adenosine receptors are proteins that reside in the extracellular membranes of cells. Activation of adenosine receptors plays a role in many physiological and pathological processes, such as immune responses and cancers.

Cell-based medicinal products (CBMPs), such as CAR T cells, are highly complex biopharmaceutical products offering various treatment options for serious illnesses such as cancer or autoimmune diseases.

Promotor: Prof.dr. B.R. Katzy

Promotores: Prof.dr. W.J.G.M. Peijnenburg & Dr. M.G. Vijver

Promotor: Prof.dr. M. Danhof, Co-Promotor: J. de Jongh

This monograph focuses on clustering of objects in high-dimensional data, given the restriction that the objects do not cluster on all the attributes, not even on a single subset of attributes, but often on different subsets of attributes in the data.

Promotores: W. Jiskoot, F. Ossendorp

Promotor: Jan van der Greef; Co-promotors: Mei Wang; Eduard van Wijk

Glycosidases (GHs) are enzymes responsible for the degradation of carbohydrates and play many roles in human health and pathophysiology. Often, abnormal levels of glycosidase activity are markedly linked to human pathologies.

Promotor: Prof.dr. J.G.E.M. Fraaije, Co-promotor: A. Kros

Inverse problems are problems where we want to estimate the values of certain parameters of a system given observations of the system. Such problems occur in several areas of science and engineering. Inverse problems are often ill-posed, which means that the observations of the system do not uniquely…

Building a Deep Cross-media Transfer Network to extract visual relations that relieve the problem of insufficient training data for visual tasks.

uman skin equivalents (HSEs) are in vitro 3D-skin models that mimic many aspects of the native human skin (NHS) and can be a valuable tool.

Promotor: H.S. Overkleeft, Co-Promotor: B.I. Florea