The sequence-dependence of biomolecular interactions involving nucleic acids and proteins is essential for numerous processes inside the cell. Insights into the underlying molecular mechanisms have been obtained using various biochemical and biophysical methods on two different levels — bulk and sin…

Single-molecule spectroscopy has become a powerful method for using organic fluorescent molecules in numerous applications.

The research presented in this thesis makes use of small molecules (as H2 , D2 and O2 ) on well-defined single crystal surfaces (flat Pt(111), flat Cu(211) and curved Pt(111)) to elucidate the role of surface structure and degrees of freedom in the reactant in specific surface reactions.

Single-Molecule Microscopy (SMM) techniques constitute a group of powerful imaging tools that enable researchers to study the dynamic behavior of individual molecules.

In this thesis we investigated the ability of two-photon multifocal microscopy for single-molecule microscopy in live cells and organisms.

Detecting nanoscopic objects plays an important role in nanoscience in particular, in the rapidly growing field of nanobiology. The forebear to modern super-resolution microscopy for single molecule investigation, is fluorescence microscopy.

This thesis introduces the concept of

This thesis is a collection of experimental attempts to enhance photoluminescence of fluorescent molecules and quantum dots with single gold nanorods (GNRs) and relevant applications.

Atoms and molecules are the basic units of matter. If we keep dividing a bar of gold or a glass of water into smaller parts, at the end we are left with a single gold atom or a water molecule.

Hoe beinvloed de structuur van één enkel gen de activiteit van genen die gevoelig zijn voor hormonen?

Tracing the evolution of the molecular gas content in galaxies is critical for a complete understanding of galaxy formation and evolution, as it provides the direct fuel for star formation. Studies of high-redshift (z>1) molecular gas reservoirs, most commonly traced by carbon monoxide (CO), have seen…

This thesis aims to improve the detection from ultra-weak single emitter by enhancing their emission properties with plasmonic nanostructures. We exploit the wet-chemically synthesized single crystalline gold nanorods (GNRs) as our basic frameworks in the whole studies, simply because of their unique…

Waarom Molecular Science & Technology in Leiden? Je wordt niet chemicus óf technoloog, maar beide! De opleidingsvideo vertelt je er alles over.

In this work, we investigate the minute circular dichroism effects of single nanoparticles.To this aim, we apply photothermal imaging with a polarization-modulated heating beam.

Promotores: Prof.dr. D. Bouwmeester, Prof.dr. A. Fiore (TU Eindhoven)

In this thesis, we study different curved single crystals because of the diversity of surface structures across their curvature.

This dissertation revolves around the design and implementation of novel instrumentation and related measurement techniques, at the single molecule level, for use in biophysical research. Chapter 1 presents an introduction to the field of fluorescence-based single molecule measurements. In particular,…

Promotor: J.N. Kok, P.E. Slagboom Co-promotor: J.F.J. Laros

Deze opleiding integreert genetica, moleculaire biologie en biotechnologie in een aantrekkelijk programma met uitstekende carrièreperspectieven.

Hoe kunnen we chemische processen nog efficiënter maken? Hoe ziet ons gebruik van brandstoffen eruit in de toekomst? Kunnen we door middel van de kennis van de chemie van de mens nog betere medicijnen maken? Zullen we ooit ziektes compleet kunnen uitbannen?

Spectroscopic studies on fluorescent single molecules in organic condensed matter does not only provide information about the molecule itself, but also its near environment. By suppression of phonon-induced broadening of spectral lines through cooling to low temperatures, small changes in the spectral…

The adsorption of D2 and CO2 on catalyst surfaces is studied using a molecular beam in ultra-high vacuum.

Promotor: Prof.dr. G.J. Kroes

Promotor: T. Schmidt, Co-promotor: S. J.T. van Noort

Bij de bacheloropleiding Molecular Science & Technology aan de Universiteit Leiden ontwikkelen studenten een sterke theoretische basis in de scheikunde en doen ze kennis van de technologische toepassingen op. Daarnaast leren studenten een bijdrage te leveren aan vraagstukken op het gebied van duurzaamheid,…

Molecular complexes can be used as electrocatalysts for oxygen reduction, water oxidation, and/or hydrogen peroxide production.

Metal complexes and 2D materials like graphene were combined to produce structures that can function as sensors.

Promotores: Prof.dr. G.W. Canters, Prof.dr. T.J. Aartsma

Promotor: Prof.dr. M.A.G.J. Orrit

Curved single crystals provide variable, but well-defined surface structures.

Promotor: Thomas Hankemeier, Co-promotores: Heiko van der Linden, Paul Vulto

In this thesis, we describe the potential of cell mechanical phenotyping for immune cell characterization, diagnosis, drug testing, as well as treatment of disease.

In human cells, a meter-long DNA is condensed inside a micrometer-sized cell nucleus. Simultaneously, the genetic code must remain accessible for its replication and transcription to functional proteins.

Deze opleiding biedt de mogelijkheid om onderzoek op moleculair, cellulair en organismaal niveau te verenigen, maar biedt ook de mogelijkheid om je te specialiseren op één van deze niveaus.

Ingen

With the energy transition toward a renewable energy supply and a CO2-neutral economy, electrification of the energy system is rising in importance, which leads to the challenge of long-term storage of renewable electricity.

This thesis provides an overview of the development of the Pair Configuration to Molecular Activity Coefficient (PAC-MAC) model.

Molecular materials have been a subject of interest in fundamental research and applications for decades, and have been studied as bulk crystals, (thin) films and as individual molecules, due to the large variety in their properties. This dissertation explores pentacene crystals near the two-dimensional…

Phosphorylation affects all four major biomolecules – proteins, lipids, carbohydrates and nucleic acids – and plays a pivotal role in the most fundamental cellular functions.

A single self-assembled semiconductor quantum dot in a high-finesse optical microcavity - the subject of this thesis - is an interesting quantum-mechanical system for future quantum applications. For instance, this system allows trapping of an extra electron and thus can serve as a spin quantum memory,…

Gold nanorods are biocompatible nanoparticles that present an excellent two-photon signal that can be used to get high spatial resolution inside living cells. Gold nanorods are photostable and therefore can be followed inside cells for long time, with possible applications as trackers in live cells.

Early time-resolved experiments by Frauenfelder on the ensemble of the kinetic rebinding of CO to myoglobin molecules resulted in a stretched exponential relaxa-tion due to a very large spread of the reaction rates of individual molecules.

Promotor: Prof.dr. H.V.J. Linnartz, Co-Promotores: S. Ioppolo, H.M. Cuppen

Promotor: M.A.G.J. Orrit

Before the large scale use of renewable energy sources can be implemented in our society, the storage of electrical energy needs to be tackled. Storage the energy as hydrogen via the reduction of protons is a good option.

Promotor: G.F.A. Kersten, W. Jiskoot, Co-promotor: B. Metz

Acute cardiovascular syndromes, including myocardial infarction or stroke, are the principal cause of death in the Western society. The main underlying pathology of cardiovascular diseases is atherosclerosis, which is caused by the accumulation of lipids and inflammatory cells in the vessel wall, in…

The research described in this thesis has, using the zebrafish as a model system, shed new light on the intricate relationship between TB and DM2, in particular on the role of leptin, SHP-1 and glucocorticoids.

This thesis consists of two main parts. The first part (Chapters 2 and 3) deals with a class of Markov process called Quasi-Skipfree (QSF) processes.