The deeper understanding of an organism's pathology is important for developing treatments. Over centuries of systematic research, clinical researchers have demonstrated that the more information they acquire about the cellular properties and their organisation in the tissue, the better they can understand…

This thesis describes the development and validation of new high-contrast imaging techniques, with the ultimate goal of enabling the next generation of instruments for ELT-class telescopes to directly image Earth-like extra-solar planets orbiting around nearby stars.

The next generation of high-contrast imaging instruments on space-based observatories requires sophisticated wavefront sensing and control in addition to a high-performance coronagraph.

In this thesis, bioorthogonal chemistry is combined with correlative light-electron microscopy to selectively label and study pathogenic intracellular bacteria within the host immune cell.

G protein-coupled receptors (GPCRs), one of the largest families of membrane proteins, are responsive to a diverse set of physiological endogenous ligands including hormones and neurotransmitters.

Understanding DNA repair mechanisms is essential for advancing plant biotechnology and crop improvement. This study investigates how double-strand breaks (DSBs), induced by CRISPR/Cas systems, are repaired in the model plant Arabidopsis thaliana, and how these processes influence Agrobacterium tumefaciens…

Heart failure is a major health care problem with high mortality.

Promotor: Prof.dr. W.J.G.M. Peijnenburg

As a non-invasive genetic method, eDNA based approaches have become an important component of ecologists' and environmental managers' toolkits for biomonitoring in conservation and an increasingly important source of ecological knowledge.

Kinase inhibitors are key therapeutic agents, particularly in oncology, yet their clinical efficacy is often hampered by off-target effects and limited understanding of their cellular target profiles.

Ben jij benieuwd waarom mensen zijn zoals ze zijn – en hoe ze veranderen? Bij de Universiteit Leiden duik je in maatschappelijk gedrag, ideeën en culturen, van vroeger tot nu en van lokaal tot wereldwijd. Van grote steden tot wereldreligies, van filosofische vragen tot opvoeding en onderwijs – je onderzoekt…

Advanced mass spectrometry of glycosphingolipids takes the central stage in this thesis. Investigations focus on characterization of glycosphingolipid metabolism in health and disease with emphasis to the detection and accurate quantitation of known and so far unknown glycosphingolipids and closely…

A New Technology-Based Firm (NTBF) is a significant enabler of job creation and a driver of the economy through stimulating innovation.

Promotores: T. Hankemeier; R. Berger, Co-promotor: R.J. Vreeken

In this thesis, the focus lies on studying glucocorticoid receptor dynamics in living cells with the aim of understanding how this transcription factor finds its DNA target sites to regulate transcription.

The spindle-assembly checkpoint (SAC) is a safety mechanism which secures accurate chromosome segregation during mitosis.

The effective treatment of tuberculosis (TB) remains a major challenge to global health.

Traditional drug discovery approaches have been hampered by (in vitro) cell-culture models that poorly represent the situation in the human body.

Promotor: J. Aarts

The main goal of the research described in this thesis was the development of new photoactivated chemotherapy (PACT) ruthenium(II) complexes bearing a non-toxic photolabile ligand.

In today's volatile market environments, companies must be able to continuously innovate. In this context, innovation does not only refer to the development of new products or business models but often also affects the entire organization, which has to transform its structures, processes, and ways of…

Streptomyces present a valuable platform for natural product discovery. Lugdunomycin is a novel angucycline-derived polyketide from Streptomyces sp QL37, with unprecedented skeleton and antimicrobial activity.

One of the main challenges in the development of an effective anti-cancer vaccine is the generation of an adequate and directed cellular immune response.

De programmagroep Onderwijswetenschappen verzorgt onderwijs op het gebied van onderwijs, het leren en de ontwikkeling van leerlingen in de schoolcontext. Studenten worden aangemoedigd uitkomsten van wetenschappelijk onderzoek te gebruiken om hun mening over het onderwijsbeleid en de onderwijspraktijk…

Biopolymer sequencing with graphene edge-based tunnel junctions has the potential to overcome current limitations with the third generation of sequencing based on biological nanopores.

Promotors: C.A.J. Knibbe, Anthonius de Boer, Co-promotor: M.M.J. van der Vorst

Streptomyces are prolific producers of enzymes and secondary metabolites, including more than 50% of all clinical antibiotics. This makes them highly attractive for medical, biotechnological, and industrial purposes.

Promotor: G.P. van Wezel, Co-promotor: D. Claessen

Humans and songbirds learn their vocalizations early in life by exposure to the vocalizations of adult conspecifics. Often, better learning outcomes are achieved with live, social, tutors than with audio-only exposure to vocalizations.

This thesis utilises the exceptional sensitivity and resolution of LOFAR to explore the low-frequency Universe, while simultaneously advancing the telescope's capabilities to enable new scientific discovery.

Viral hemorrhagic fever (VHF) is a group of acute diseases caused by highly infectious viruses including Ebola, Lassa, Dengue viruses. Its high mortality rate poses high risk to public health, however, studies on VHF have been hampered due to the non-availability of proper models and incomplete knowledge…

Inflammatory skin diseases such as psoriasis and atopic dermatitis can severely impact both the physical and social aspect of a patient’s life. Aberrant inflammatory responses are thought to be a major causative factor in these disorders, but an abundance of different factors contribute to the development…

Wat zijn de beste werkwijzen om studenten te leren omgaan met complexe problemen?

This thesis investigates a pharmacological challenge model induced by Toll-like receptor 7 (TLR7) activation, focusing on skin inflammation and its implications for early drug development.

Promotor: M. Danhof, Co-promotor: E.C.M. de Lange

Assessing the risks of engineered nanomaterials (ENMs) solely on the basis of experimental assays is time-consuming, resource intensive, and constrained by ethical considerations (such as the principles of the 3Rs of animal testing). The adoption of computational toxicology in this field is a high p…

The department of Industrial Ecology is dealing with research into the development of tools for decision making on sustainable production and consumption.

Electron microscopy has become an extremely important techniquein a wide variety of elds.

One of the key limitations of the direct imaging of exoplanets at small angular separations are quasi-static speckles that originate from evolving non-common path aberrations (NCPA) to which the primary adaptive optics system is inherently blind. The main focus of this thesis is the development and…

In most applications, electrocatalysts exhibit a large surface area to volume ratio, for example using nanoparticles.

β-Lactamases are enzymes that can break down β-lactam substrates, such as antibiotics, preventing the use of these antibiotics for the treatment of various infectious diseases. However, some compounds, β-lactamase inhibitors, can block these enzymes allowing for possible treatments using a combination…

The skin is our natural barrier and lipids are a key part of this barrier. In the outer skin layer, the stratum corneum (SC), lipids form a densely organized structure dependent on the composition of these lipids.

In deze bundel wordt verslag gedaan van onderzoek op het gebied van duurzaamheid, uitgevoerd aan de Leidse rechtenfaculteit.

Het Leiden Leadership Centre doet onderzoek naar de ontwikkeling van doeltreffend publiek leiderschap binnen en tussen organisaties. Op basis van onze onderzoeks- en ontwikkelagenda “Verder met Publiek Leiderschap” richten we ons op publiek leiderschap in verbinding met de publieke context, maatschappelijke…

This thesis explores how electron paramagnetic resonance (EPR) spectroscopy can be used to investigate key biochemical processes.

Sinds de start van de Green Office zijn er verschillende projecten gestart om de bewustwording onder medewerkers & studenten te vergroten en de universiteit te verduurzamen. Lees hier alles over onze huidige projecten.

Promotor: Prof.dr. B.R. Katzy, Prof.dr. J. de Vries

Promotor: S.M. Verduyn Lunel

The elementary excitations of magnets are called spin waves, and their corresponding quasi-particles are known as magnons. The rapidly growing field of Magnonics aims at using them as information carriers in a new generation of electronic devices, (almost) free of electric currents.

Radiography is an important technique to inspect objects, with applications in airports and hospitals. X-ray imaging is also essential in industry, for instance in food safety checks for the presence of foreign objects.