Scattering of light in the presence of nano-structured materials, i.e. with features in the order of the wavelength of the light or smaller, reveals details of how light interacts with matter at the nanoscale.

The main aim of this thesis was to unravel the signaling and regulatory networks that drive tumor cell migration during breast cancer metastasis.

This dissertation contains four works during my PhD. Different biomaterials have been designed based on coiled-coil peptides. These biomaterials have a range of applications, inclusing drug delivery, cell sorting to cell-cell fusion.

Viral hemorrhagic fever (VHF) is a group of acute diseases caused by highly infectious viruses including Ebola, Lassa, Dengue viruses. Its high mortality rate poses high risk to public health, however, studies on VHF have been hampered due to the non-availability of proper models and incomplete knowledge…

Afscheidscollege

Carbohydrates are present on the surface of bacteria making them suitable antigen candidates for vaccine development. This thesis deals with the synthesis of two carbohydrate-based components; the capsular polisaccharide of S. aureus type 5 and teichoic acids from staphilococci and enterococci speci…

In this thesis, the focus lies on studying glucocorticoid receptor dynamics in living cells with the aim of understanding how this transcription factor finds its DNA target sites to regulate transcription.

The overall theme of this thesis is to complement the electrochemical data acquired in fuel cell research with in situ X-ray photoelectron spectroscopy (XPS) and soft X-ray absorption spectroscopy (XAS).

Particles are omnipresent in biopharmaceutical products. In protein-based therapeutics such particles are generally associated with impurities, either derived from the drug product itself (e.g. protein aggregates), or from extrinsic contaminations (e.g. cellulose fibers).

Details kunnen zien van een zieke of gezonde cel, dat is mogelijk met de supermicroscoop van het consortium Cyttron. Niet alleen de microscoop is vernieuwend in zijn soort: ook de vorm waarop de partijen samenwerken past in de huidige tijd. “Toegang tot elkaars techniek, daar draait het om in ons samenwerkingsverband”,…

Met de nodige maatregelen starten onderzoekers hun werkzaamheden in verschillende laboratoria weer deels op. Het Leidse Cell Observatory is één van de plekken waar wetenschappers sinds 11 mei hun labwerk hervatten.

Promotor: Prof.dr. T. Schmidt

Nederlandse politicologen laten hun licht schijnen over het veranderende electoraat.

Promotor: G.A. van der Marel, Co-promotor: J.D.C. Codée

Healthy aging is one of the prime goals in today's society and atherosclerosis is among the greatest causes of morbidity in elderly. Cardiovascular disease patients receiving treatment are often of advanced aged and have an aged immune system, which limits translating experimental findings to the pa…

In this thesis, the role of mast cells in atherosclerosis and novel therapeutic strategies to inhibit atherosclerosis progression are discussed. The first part of the thesis specifically focuses on the relation between mast cells and advanced human atherosclerotic plaque characteristics.

Atherosclerosis is the most prominent underlying pathology of cardiovascular disease and an important cause of major adverse cardiovascular events.

Promotor: Prof.dr. J. Kuiper

Promotor: Prof.dr. H.P. Spaink, Co-promotor: Dr. B.E. Snaar

Promotor: B. vd Water, T. Schmidt, Co-Promotor: E.H.J. Danen

Promotors: W. Jiskoot; G.J. Witkamp, Co-promotor: M.R. Nejadnik

Promotores: Prof.dr. H.P. Spaink & Prof.dr. P.C.W. Hogendoorn Co-promotor: Dr. M.J.M. Schaaf

Promotores: B. van de Water; P. Hogendoorn; J. Bovée Co-Promotor: E.H.J. Danen

Decentralized plug and play systems for energy production are the future picture of our society. Artificial photosynthetic systems are used for this purpose.

Promotores: H.S. Overkleeft, G.A. van der Marel, Co-Promotor: R.G. Boot

This thesis describes our search to identify and understand possible regulatory mechanisms of integrin α6β4 in cell-matrix adhesion and intracelular signaling.

Op 25 september promoveert Alzenir Mendes Martins de Menezes. Het Leiden University Centre for Linguistics feliciteert Alzenir!

Promotor: Roeland M.H. Merks

In this thesis, the researcher developed a nanosystem based on the metallophilic Interaction between cyclometalated complexes.

Streptomyces present a valuable platform for natural product discovery. Lugdunomycin is a novel angucycline-derived polyketide from Streptomyces sp QL37, with unprecedented skeleton and antimicrobial activity.

Bacterial biocatalysis constitutes a sustainable alternative for high-value chemicals production by enabling the utilization of renewable feedstocks.

This dissertation combines the use of defined microenvironments, high-resolution fluorescence microscopy, and time-resolved analysis, to study intracellular and cellular motility.

Promotor: G.P. van Wezel, Co-Promotor: E. Vijgenboom

Promotor: J.G.E.M. Fraaije, Co-Promotor: A. Kros

Promotores: J. Jonkers, K.E. de Visser

The human body consists of hundreds, perhaps thousands of different types of cells, each with different morphologies and functions, despite having the same genome.

Reducing greenhouse gas emissions is one of the most pressing challenges that humanity faces in the coming decades.

In summary, the collective results described in this thesis show that nanoparticulate vaccines can be delivered intradermally by coated and hollow microneedles and evoke antigen-specific immune responses.

Promotor: A.P.IJzerman Co-promotor: A. Bender

Deze zomer verruilen de jonge Leidse politicologen Feike Fliervoet en Gerrit Krol hun vertrouwde bestaan als werkgroepdocent voor een onderzoeksplek in Florence. Na vele jaren te hebben gewerkt als student en vervolgens docent bij Politieke Wetenschap, worden zij promovendus aan het gerenommeerde European…

Over de sporen die Darwin, hoewel nooit in Leiden geweest, er toch achterliet.

The present thesis focuses on the pharmacological concept of drug-target interaction, which dates back to the beginning of modern pharmacology.

In eight chapters, the thesis of Rafaël Vos covers the effect of temperature and pressure on the electrochemical CO2 reduction.

This thesis describes the synthesis and biological evaluation of TLR2/6, TLR4, TLR7/8 and TLR9 ligands, of which the activity can be conditionally controlled.

Promotor: A. Kros, Co-promotor: R.R.C.L. Olsthoorn

The main goal of the research described in this thesis was the development of new photoactivated chemotherapy (PACT) ruthenium(II) complexes bearing a non-toxic photolabile ligand.

Despite the fact that gold nanoparticles (GNPs) are one of the most studied nanoparticles, there is still a necessity for new approaches allowing for effective protective coating to enable wider use of GNPs in biomedical applications.

In this thesis, we aimed to better understand the underlying mechanisms involved in TNBC progression and metastasis formation and discover new targets to reduce breast cancer related deaths.