The research described in this thesis centered on retaining exo-β-glucosidases implicated in health and disease, in particular the human inherited lysosomal storage disorder, Gaucher disease (GD).

This dissertation explores how Large Language Models (LLMs) can effectively and responsibly contribute to complex decision-making processes. By combining AI and human intelligence, Hybrid Intelligence (HI) emerges, allowing the strengths of both humans and machines to be utilized.

International trade has played a major role in defining the modern global economy. Trade, however, entangles the environmental pressures of economic sectors, giving the illusion of environmental improvements, while the opposite may be occurring.

In the last decade, the redox interconversion between metal thiolate and disulfide compounds has been extensively investigated for copper, but not for other transition metal ions.

The effects of anthropogenic noise on interactions between predators and their prey are still little understood.

Promotores: Wim Jiskoot; Ferry Ossendorp

Promotor: Prof.dr. M.K. Richardson

This thesis describes the development and optimization of the first molecular tools to study the enzyme PLA2G4E.

Membrane fusion is a vital process in living organisms and is mediated by zipper-like proteins.

AI Labs zijn samenwerkingen van de Universiteit Leiden met externe partner zoals bedrijven, overheidsinstellingen en andere universiteit op het gebied van kunstmatige intelligentie. De Faculteit voor Wis-en Natuurkunde is uniek gesitueerd op het grootste Bioscience Park van Nederlands, en ligt direct…

This thesis describes the design, characterization and application of dextran based crosslinked network.

Promotores: G.W. Canters, T.J. Aartsma

Agrobacterium tumefaciens, a gram-negative plant pathogen belonging to the family Rhizobiaceae, is the causative agent of crown gall disease, which can affect many plant species including agronomically important ones.

Promotor: G.A. van der Marel, Co-promotor: J.D.C. Codée

With the help of quantum mechanics, digital quantum hardware may be able to tackle some of the problems that are too difficult for ordinary computers. But despite these expectations and the ongoing effort of the research community, reliable quantum computers are not yet realized in a lab setting.

Promotor: J. Zaanen

Promotor: Prof.dr. F.S. de Boer, Co-promotor: D. Clarke

Climate change is one of the largest challenges faced by humanity. To combat this research into alternatives to fossil fuels is ongoing. Dihydrogen is considered a good alternative fuel, since its burning only forms water.

Continuous optimization is never easy: the exact solution is always a luxury demand and the theory of it is not always analytical and elegant.

Promotor: Prof.dr. C.W.J. Beenakker

Catalysis is of extreme relevance in the production of everyday materials and plays a central role in many aspects of our life.

Promotor: Koen Kuijken Co-promotor: Henk Hoekstra

Promotor: M.K. Richardson, Co-Promotor: F. Witte

The lysosomal β-glucosidase named glucocerebrosidase (GCase) is a retaining β-glucosidase that hydrolyzes the glycosphingolipid glucosylceramide (GlcCer) to ceramide and glucose at acid pH.

Promotor: J.N. Kok, Co-Promotor: A.J. Knobbe

Promotores: T.H.W. Bäck, Y. Tan, Co-promotor: M.T.M. Emmerich

This PhD thesis concerns the topic of arithmetic geometry. We address three different questions and each of the questions in some way is about counting how big some set is or can be.

Promotor: Prof.dr. B.R. Katzy, Prof.dr. H.J. van den Herik, Prof.dr. G.H. Baltes (University of Applied Sciences Konstanz)

Promotores: Prof.dr. G.A. van der Marel, M. van Heel

Mining time series is a machine learning subfield that focuses on a particular data structure, where variables are measured over (short or long) periods of time.

Promotores: J. Jonkers, K.E. de Visser

Galaxies are environments where gas coalesces, cools, and is converted into stars. However, it remains unclear the exact mechanisms through which galaxies acquire, redistribute and lose their gas.

Lysosomal acid glucosylceramidase (GBA1) is a lysosomal enzyme that degrades glucolipids with its main substrate being glucosylceramide (GlcCer). Defects in the GBA1 gene lead to glycosphingolipidosis Gaucher disease (GD), in which the hydrolysis of GlcCer is impaired and therefore, it accumulates in…

Promotors: Prof.dr. P.J. Punt, Prof.dr. C.A.M.J.J. van den Hondel

During my PhD research, I studied the photon statistics of light emitted by a microcavity that contains a single quantum dot (QD) on resonance.

Promotor: S.M. Verduyn Lunel

All life on earth contains DNA, which is used to store biological information. Organisms compact their DNA in order for it to fit inside their cell(s).

Promotor: M. Danhof, Co-promotor: E.C.M. de Lange

Shape and biological function are tightly connected. Physical descriptions are used to connect the shape of a biological system with its function.

The work described in this dissertation contributes to a better mechanistic understanding of nanoparticles in vivo. To achieve that goal, we used the zebrafish as a highly predictive pre-screening model of nanoparticles.

Promotor: J.N. Kok, P.E. Slagboom Co-promotor: J.F.J. Laros

Anthropogenic noise has been shown to affect marine animals in various ways, this may have fitness consequences at individual and population level. This thesis aims to increase insight into the quantification of sound-induced behavioural responses that are relevant to fitness, and into factors that…

The electrochemical oxygen reduction reaction (ORR) is an essential half-reaction for the utilization of hydrogen as a sustainable fuel, via the conversion of hydrogen to electrons and protons facilitated by the ORR. In the most common fuel cells, the ORR is requires high loadings of non-abundant platinum…

In my research, we developed a method to distinguish knots. A knot is a mathematical depiction of the everyday knot that occurs in ropes and cords.

Assessing the risks of engineered nanomaterials (ENMs) solely on the basis of experimental assays is time-consuming, resource intensive, and constrained by ethical considerations (such as the principles of the 3Rs of animal testing). The adoption of computational toxicology in this field is a high p…

The power of personalized nutrition lies in being able to conduct clinical research on healthy people while capturing metabolic markers sensitive to the impact of environmental and metabolic stressors (e.g. diet, changing sex hormones and the menstrual cycle).

Het doel van het RAISE Onderzoek is om te onderzoeken hoe veerkrachtige mensen omgaan met stress. We kijken specifiek naar hoe belangrijke biologische systemen, zoals de HPA-as en het immuunsysteem, samenwerken met de hersenen en de sociale omgeving om veerkrachtig functioneren na moeilijkheden in de…

In this thesis we explore machine and deep learning approaches that address keychallenges in high dimensional problem areas and also in improving accuracy in wellknown problems. In high dimensional contexts, we have focused on computational fluid dynamics (CFD) simulations.

Producing green energy has become the main goal in our society in the search of reducing or eliminating the carbon emission from fossil fuels.