Chapter 2 describes a post-synthetic methodology to introduce a fluorescent label in highly lipophilic, Pam3Cys based conjugates.

Voor de omzetting van zonlicht naar brandstof

This thesis describes the use of an activity-based proteomics method to study the endocannabinoid system.

To this day, all cyclophellitol-based inhibitors and ABPs have been close analogues of their natural substrate counterparts. As a result, these probes showed high selectivity towards their target glycosidases.

Climate change is a challenge for both current and future generations. New biological resources have to be developed in order to meet the demand for energy as well as the demand for food.

In the search for sustainable energy solutions, the idea of artificial photosynthesis has been proposed as an approach with which to use water and sunlight to produce hydrogen.

Less than 1 in 10 drug candidates that enter phase 1 clinical trials actually gets approved for human use.

Large parts of the research described in this Thesis aims at the development of oligopeptide-masked toxins and their in situ immunoproteasome-mediated activation.

In this thesis, the discovery and optimization is described of chemical tools to study the N-acylethanolamine (NAE) biosynthetic pathway.

Promotor: Prof.dr. H.J.M. de Groot, Co-promotor: Francesco Buda

Sander van Kasteren wilde via een simpele chemische aanpassing de werking van het immuunsysteem beter onderzoeken. Wat hij deed was het systeem platleggen. Niet de bedoeling, maar geen nood. De mislukking bracht hem op het spoor van een moleculaire schakelaar. En het leverde een hot paper op.

Promotor: H.V.J. Linnartz

This thesis focuses on the design and synthesis of activity-based probes and inhibitors targeting enzymes involved in the degradation of specific plant glycans.

Cytotoxic T-cells (CTLs) are involved in the clearance of viruses and killing of tumor cells.

Carbohydrates are present on the surface of bacteria making them suitable antigen candidates for vaccine development. This thesis deals with the synthesis of two carbohydrate-based components; the capsular polisaccharide of S. aureus type 5 and teichoic acids from staphilococci and enterococci speci…

Promotores: Prof.dr. H.S. Overkleeft, Prof.dr. G.A. van der Marel

Promotor: Prof.dr. G.A. van der Marel, Co-Promotor: J.D.C. Codée

The inverse electron demand Diels-Alder (IEDDA) pyridazine elimination emerged in 2013 as a new bioorthogonal reaction and constitutes a prime example of what is now known as dissociative bioorthogonal chemistry. The research described in this Thesis aims to develop synthetic strategies which enable…

The primary goal of this research is to deepen our understanding of substrate-enzyme interactions in Glycoside hydrolases. For this purpose, Bacillus circulans xylanase (BcX) has been selected as a well-established model to examine the Michaelis complex and glycosyl-enzyme adduct.

Chemici, waaronder Sander van Kasteren en Hermen Overkleeft, hebben ontdekt dat azides, een bepaalde chemische verbinding, specifieke T-cellen kunnen laten reageren op een vaccin of juist het vaccin kunnen laten negeren. Publicatie in Angewandte Chemie.

Light-Harvesting Complex II (LHCII) is responsible for light absorption and excitation energy transfer in plants and photosynthetic algae, while in high light it undergoes conformational changes by which it quenches excitations to prevent photodamage.

Anneleen van der Meer bespreekt in diverse media het gebruik van chemische wapens door Rusland.

Promotor: H.S. Overkleeft, J. Neefjes, Co-promotor: M. van der Stelt

Promotor: G.A. van der Marel, Co-promotor: D.C. Codée

This thesis describes the synthesis and biological evaluation of TLR2/6, TLR4, TLR7/8 and TLR9 ligands, of which the activity can be conditionally controlled.

Glycoside hydrolases (glycosidases/GHs) are widely abundant enzymes in all kingdoms of life and are important biocatalysts that catalyze the hydrolysis of glycosidic linkages in oligo/polysaccharides, glycoproteins and glycolipids with tremendous efficiency

Photocatalysis has emerged as a powerful tool for enabling challenging transformations in organic synthesis. This thesis explores the development of several photocatalytic reactions designed to form carbon-heteroatom bonds from less-functionalized precursors.

This thesis aimed to investigate core cross-linked polymeric micelles (CCPMs) and expand their potential for the delivery of hydrophobic drugs and co-factors.

In order to be able to develop effective medicine and treatments to prevent or cure autoimmune diseases or cancer we need to understand the mechanisms how they arise and what drives their course.Unravelling the fundamental molecular mechanisms influencing the onset and course of diseases such as allergies,…

Trillende koolstofmonoxide moleculen die zich binden aan het oppervlakte van een zoutkristal, stoppen binnen enkele milliseconden met bewegen. Wetenschappers hebben nu ontdekt dat dit voornamelijk komt door de emissie van elektromagnetische golven. De rol van de chemische binding met het oppervlakte…

Mirjam Luijten, toxicoloog bij het RIVM, gaat per 1 juli aan de slag als bijzonder hoogleraar bij het LACDR.

Drug development is a time- and resource-consuming process that starts with the discovery and validation of a (protein) target that contributes to pathogenesis or disease progression.

Solar energy harnessed by plants and algae has great potential to be converted into biofuels for future generations.

Phosphorylation affects all four major biomolecules – proteins, lipids, carbohydrates and nucleic acids – and plays a pivotal role in the most fundamental cellular functions.

This thesis is concerned with the constitution of chiral responsive matrix assemblies that may undergo light-driven conformational changes and self-select vibrations to develop vibronic states for driving semi-classical coherent transfer with nearly 100% yield in a lossless NCAP process.

Microbial rhodopsins are photosensitive pigments implemented in the growth and adaptation of a large population of microorganisms.

In the current global context, there is a pressing need to address sustainable energy supplies to safeguard our Planet and its ecosystems. The choices made by human society have a significant impact on genetic evolution and climate.

Reducing greenhouse gas emissions is one of the most pressing challenges that humanity faces in the coming decades.

Climate change is one of the largest challenges faced by humanity. To combat this research into alternatives to fossil fuels is ongoing. Dihydrogen is considered a good alternative fuel, since its burning only forms water.

Inspired by natural photosynthesis, perovskite-based photoelectrochemical (PEC) systems are being developed for artificial photosynthesis, aiming to enhance solar-to-hydrogen conversion for green energy.

With increasing carbon dioxide levels in the atmosphere and their detrimental effect on the global climate, modern society needs to push for more renewable energy sources. Storing widely accessible and abundant solar energy in chemical bonds in the form of molecular fuel via artificial photosynthesis…

De Leidse hoogleraren chemie Hermen Overkleeft en Hans Aerts hebben, samen met hun collega Huib Ovaa van het Nederlands Kankerinstituut, 2 miljoen euro gekregen van NWO voor biochemisch onderzoek. Het onderzoek kan leiden tot nieuwe behandelingsmethodes voor verschillende ziektes.

In this thesis, bioorthogonal chemistry is combined with correlative light-electron microscopy to selectively label and study pathogenic intracellular bacteria within the host immune cell.

Promotores: H.S. Overkleeft, G.A. van der Marel, Co-promotor: B.I. Florea

This dissertation presents the synthesis and evaluation of antibody recognition for various capsular polysaccharide (CP) fragments of Staphylococcus aureus (S. aureus).

This dissertation explores new chemical strategies for the synthesis of self-adjuvanting peptide conjugates and immunomodulatory ligands aimed at targeted activation of the immune system.

Pseudomonas Aeruginosa is a Gram-negative bacterium which can form biofilms, increasing its resistance against antibiotics and the host immune system. Polysaccharides are an integral part of this biofilm, one of these polysaccharides is called Psl. PslG is a glycosidase, able to cleave this polysaccharide,…

Vitamin A or retinol is essential in embryonic development, the visual cycle and the immune system.

Promotores: Prof.dr. G.A. van der Marel, M. van Heel

Lysosomal acid glucosylceramidase (GBA1) is a lysosomal enzyme that degrades glucolipids with its main substrate being glucosylceramide (GlcCer). Defects in the GBA1 gene lead to glycosphingolipidosis Gaucher disease (GD), in which the hydrolysis of GlcCer is impaired and therefore, it accumulates in…