This thesis describes research aimed at discovering inhibitors selective for either of the two endoplasmic reticulum α-glucosidases, ER-I and ER-II, with the overarching goal of discovering new antiviral agents to treat viruses that rely on host N-glycosylation for proliferation.

Promotor: H.S. Overkleeft, J. Neefjes, Co-promotor: M. van der Stelt

This thesis presents the first synthetic peptides ADP-ribosylated on serine, threonine, tyrosine, arginine and cysteine.

The work described in this Thesis is focused on the assembly of oligosaccharide fragments derived from a fungal polysaccharide, galactosaminogalactan (GAG) and fragments of the exopolysaccharide Pel, generated by Pseudomonas aeruginosa.

M.T.M. Koper, Co-promotor: D.G.H. Hetterscheid

Sphingolipids are important membrane compounds with a variety of functions. In mammalian cells, different enzymes are involved in the metabolism of sphingolipids, but interruption of this metabolism process leads to different diseases.

This thesis aimed to investigate core cross-linked polymeric micelles (CCPMs) and expand their potential for the delivery of hydrophobic drugs and co-factors.

Promotor: M.T.M. Koper, Co-Promotor: L.B.F. Juurlink

Nicotinamide adenine dinucleotide (NAD+) is the substrate used for the introduction of the ubiquitous and highly dynamic PTM in which either one or multiple adenosine diphosphate ribose (ADPr) moieties are covalently attached to a nucleophilic side chain of an specific amino acid in the target protein…

Promotor: Prof.dr. M. Ubbink, Co-promotor: G. Siegal

In this thesis, the researcher developed a nanosystem based on the metallophilic Interaction between cyclometalated complexes.

To design the ideal water oxidation catalyst, understanding of the catalytic mechanism and decomposition pathways is essential.

Neuraminidases are enzymes that cleave glycosidic linkages of sialic acid. These enzymes are involved in influenza infections as well as in many cellular processes in mammals and micro-organisms.

Cyclic peptides are investigated as a platform to induce different orientations between various ligands.

O-Nitrobenzyl groepen worden veel gebruikt als moleculaire beschermgroepen die je met licht kunt verwijderen.

The aim of this thesis is to study cell-cell interactions and the development of an assay to explore and quantify the exchange of membrane compounds.

The studies described in this thesis deal with glycosidases, in particular alpha-galactosidases.

Promotor: E. Bouwman, Co-Promotor: S. Bonnet

Evolution acts via mutations in amino acid sequences. Substitution of essential amino acids leads to a nonfunctional protein. l.

The electrochemical hydrogenation reaction is an alternative approach for the transformation of unsaturated bonds without the use of flammable dihydrogen gas.

This thesis highlights the significant role of surface geometry and oxidation state in determining CO adsorption behavior on copper surfaces.

Promotie

Promotie

Kunnen kleine moleculen het immuunsysteem herstellen en helpen tuberculose te bestrijden door autofagie te stimuleren?

LST biedt een uniek onderwijsprogramma dat zich richt op de levende cel. De bacheloropleiding duurt drie jaar. Tijdens je eerste jaar werk je aan een stevig fundament. Daarna is er meer ruimte voor specialisatie.

Hoe gebruiken ziekteverwekkende mycobacteriën de vetten in onze cellen, en hoe kunnen we dat tegenhouden?

Kunnen suikerpatronen op cellen het immuunsysteem activeren om kanker te bestrijden? Sander van Kasteren gebruikt de beurs van de European Research Council (ERC) om te ontdekken of dit mogelijk is.

In 2050 moet de overstap naar nieuwe, duurzame energiebronnen zijn voltooid. Wetenschappers van de Universiteit Leiden verrichten vanuit diverse disciplines uniek onderzoek dat bijdraagt aan deze transitie en de reductie van CO2.

This thesis focuses on the design and synthesis of activity-based probes and inhibitors targeting enzymes involved in the degradation of specific plant glycans.

Cytotoxic T-cells (CTLs) are involved in the clearance of viruses and killing of tumor cells.

The research described in this Thesis was aimed at designing and synthesizing nature-inspired compounds as part of TB vaccine discovery.

This thesis has described novel synthetic methods to produce a variety of (glyco)peptides and their application in the study of various immunological processes.

Promotor: M. Ubbink, Co-promotor: G. Siegal

Promotors: Prof.dr. E. Bouwman, Prof.dr. E. Drent

Dissociative chemisorption is an important reaction step in many catalytic reactions.

The goal of this thesis is to understand how interfacial parameters, such as the addition of organic additives, bulk pH or metal cations of the electrolyte, affect the relevant electrocatalytic reactions, i.e. electrochemical CO2 reduction reaction or hydrogen evolution reaction.

This dissertation focused on computational methods based on first principles calculations using the Density Functional Theory (DFT) framework. Emphasis was laid on affordable methods that can provide a tradeoff between computational expense and accuracy.

Light-Harvesting Complex II (LHCII) is responsible for light absorption and excitation energy transfer in plants and photosynthetic algae, while in high light it undergoes conformational changes by which it quenches excitations to prevent photodamage.

Promotor: H.S. Overkleeft, Co-promotor: M. van der Stelt

Promotor: G. A. van der Marel, Co-promotor: D. V. Filippov

This thesis provides an overview of the development of the Pair Configuration to Molecular Activity Coefficient (PAC-MAC) model.

Promotores: H.S. Overkleeft, G.A. van der Marel, Co-Promotor: J.D.C. Codee

Promotor: Prof.dr.ir. J.G.E.M. Fraaije, Co-promotor: A. Kros

Heterogeneous catalyzed processes are highly significant to the chemical industry. Dissociative chemisorption (DC) of molecules on surfaces is always considered as a step with a high degree of rate control for heterogeneous catalysis.

Carbohydrates are present on the surface of bacteria making them suitable antigen candidates for vaccine development. This thesis deals with the synthesis of two carbohydrate-based components; the capsular polisaccharide of S. aureus type 5 and teichoic acids from staphilococci and enterococci speci…

Promotores: Prof.dr. H.S. Overkleeft, Prof.dr. G.A. van der Marel

An objective of this research was to achieve direct, well-defined and non-rate-limiting electron transfer between respiratory enzymes and the electrode surface by means of 'Q-wires'.

Glycosidases are important enzymes in the turnover of polysaccharides and glycoconjugates, and are involved in a range of human pathologies including genetic disorders such as Gaucher and Pompe disease, but also in various cancers.

Promotor: Prof.dr. H.J.M. de Groot, Co-promotor: Francesco Buda