In this thesis, we used genetically engineered mouse models and a variety of cell-culture based assays to identify genes and pathways that are involved in the development and treatment of invasive lobular carcinoma (ILC).

Promotie

Promotor: A.P. IJzerman Co-promotor: L.H. Heitman

Over several decades, a variety of computational methods for drug discovery have been proposed and applied in practice. With the accumulation of data and the development of machine learning methods, computational drug design methods have gradually shifted to a new paradigm, i.e. deep learning methods…

Behandelmethoden voor chronische ziekten zijn beperkt. Artsen en onderzoekers van het Leids Universitair Medisch Centrum (LUMC) en de Universiteit Leiden werken aan nieuwe therapieën als alternatief voor orgaantransplantatie. Doel is om organen te herstellen in hun oorspronkelijke functie en op die…

The research described in this thesis focused on identifying novel drug targets and synergistic combinations for triple-negative breast cancer (TNBC), a virulent subtype of breast cancer with a dismal prognosis and limited therapeutic options.

Shape and biological function are tightly connected. Physical descriptions are used to connect the shape of a biological system with its function.

Solute carrier (SLC) transporters are a large and diverse class of relatively understudied transmembrane proteins.

Tijdens de viering van de 54ste Dies Natalis van Universitas Padjadjaran in Bandung, Indonesië, zijn op 9 september 2011 onderscheidingen uitgereikt aan de Leidse prof.dr. L.J. Slikkerveer en drs. L.E.H. Vredevoogd. Zij kregen deze voor hun uitzonderlijke bijdrage aan de succesvolle wetenschappelijke…

Drug development critically depends on preclinical models that mimic human physiology, yet traditional two-dimensional cultures and animal models often lack predictive power.

Promotor: B. van de Water

Mechanical forces are known to play an important role in bacterial colonies. In this dissertation, we study the self-organization at various stages of growing bacterial colonies, and focus on the mechanical effects of cell growth.

Cardiovascular diseases are a leading cause of death worldwide, and better models are urgently needed for disease progression studies and drug development.

This thesis investigates how cell shape and collective behavior influence the dynamics and structure of biological tissues, which are crucial for understanding processes like embryonic development, tissue regeneration, and metastasis.

Promotor: B. van de Water

Image-Pro Plus, ImageJ, Cell Profiler, R, Knime en NIS-elements

Promotor: Huub J.M. de Groot, Co-promotor: Francesco Buda

De nieuwe software van Microsoft, VALL-E hoeft maar drie seconden van een stem te horen om hem bijna foutloos te kunnen imiteren. Je kunt zelf kiezen welke emotie de stem moet gebruiken en wat hij moet zeggen. Valerie Frissen, Hoogleraar Digitale Technologie en Sociale Verandering bij eLaw en directeur…

Integrins play an essential role in multicellular life by connecting cells to the extracellular matrix.

Endothelial dysfunction and microvascular diseases are key drivers of cardiovascular disease, the world's leading cause of death.

The first steps are made in personalised cancer vaccination strategies, which aim to induce a more specific immune response with fewer side effects. The activation of cytotoxic T cells is crucial for an effective immune response.

The overarching goal of this thesis is to set the foundations, but also make the first essential steps towards establishing a comprehensive, mesoscopic, hydrodynamic theory of epithelial tissues. The stage is set by an exhaustive study of topological defects in passive p-atic liquid crystals, singularities…

Image segmentation is a fundamental task in computer vision, with applications ranging from medical diagnostics to archaeological research.

Cholesterol influences many pathways, including serving as precursor for adrenal steroidogenesis. Imbalance of cholesterol levels has been implicated in several diseases including cardiovascular diseases and its underlying pathology, atherosclerosis.

In photoactivated chemotherapy (PACT), a biologically active compound is caged by a light-cleavable protecting group.

Pericytes, the mural cells of blood microvessels, are important regulators of vascular morphogenesis and function that have been postulated to mechanically control microvascular diameter through as yet unknown mechanisms.

In this thesis, we focus on novel proteins/mechanisms that regulate integrin-adhesion mediated mechano-transduction.

This thesis focuses on the synthesis, characterization and performance towards CO2 electroreduction of mono and bi-metallic particles based on p-block metals

In previous studies at our laboratory it was demonstrated that drug exposure of HepG2 cells can lead to an altered TNFα-induced NF-κB oscillatory phenotype, concurrent with a synergistically increased sensitivity for TNFα-induced apoptosis.

This doctoral thesis is an effort to understand how lipid phase-separation induced by diacylglycerol analogues in lipid-based nanoparticles affects their in vivo behavior, leading to specific nanoparticle-protein communications and selective cell targeting.

De keten die patiënten voorziet van nieuwe medicijnen, bestaat uit diverse complexe schakels: van fundamentele wetenschap, tot klinische tests, tot productie. Deze hele keten is aanwezig in Leiden. De Universiteit Leiden, het Leids Universitair Medisch Centrum (LUMC) en de bedrijven op het Leiden Bio…

Wat is het persoonlijke verhaal van wetenschappers als Einstein, Kamerlingh Onnes en Swammerdam? Een aantal Leidse honoursstudenten heeft een virtuele tentoonstelling opgezet om de mensen achter de ontdekkingen weer te geven.

Promotie

This thesis describes the synthesis, analysis of various tetrazine bearing compounds and use in bioorthogonal chemistry.

The molecular mechanisms that instigate a healthy cell to become malignant are fueled by (epi)genetic alterations in so-called driver genes.

In human cells, a meter-long DNA is condensed inside a micrometer-sized cell nucleus. Simultaneously, the genetic code must remain accessible for its replication and transcription to functional proteins.

Promotores: Prof.dr. W.J.G.M. Peijnenburg & Dr. M.G. Vijver

The applications of studying the early development of insects range from agriculture to material science.

Promotor: H.P. Spaink, Co-Promotor: A.H. Meijer

The function of TLRs in innate immunity has aroused worldwide attention soon after its discovery. Because of the broad functions of TLR2 in innate immunity, the drive for the development of TLR2-targeted vaccines or therapeutic treatments has accelerated in the last decades.

Promotor: Johan Kuiper, Co-promotor: Saskia C.A. de Jager

Promotores: Prof.dr. H. van Veen & Prof.dr. W. de Boer (Wageningen University)

Promotor: J. Kuiper

This thesis tackles the difficulty of predicting the liver’s clinical response to novel therapies, a challenge arising from interspecies differences and limitations of existing in vitro models.

The type-IV secretion system (T4SS) is a machinery able to transfer DNA and proteins between bacteria and in certain cases also to eukaryotic cells.

Promotores: A.H. Meijer, H.P. Spaink

Breast cancer is the most common cancer in women worldwide. Metastasis, the process by which cancer cells spread to other parts of the body, makes treatments less effective. It is important to unravel the mechanism of metastasis.

The investigations described in this thesis lay out strategies aimed at advancing antibiotic research and development. The examples presented revolve around two main approaches: understanding drug-target interactions and target identification.

This thesis describes the functional analysis of the Arabidopsis AHL15 gene.