This thesis tackles the difficulty of predicting the liver’s clinical response to novel therapies, a challenge arising from interspecies differences and limitations of existing in vitro models.

Promotor: J. Kuiper

Promotor: J. A. Bouwstra

Promotor: Prof.dr. T. Hankemeier

Dit project ontwikkelt een wetenschappelijke methode om klinisch relevante nieuwe informatie te extraheren uit patiëntenforum-websites die ervaringen van patiënten bespreken over bv. medicatie, voeding, co-morbiditeiten, genetische factoren enz.

Traditional drug discovery approaches have been hampered by (in vitro) cell-culture models that poorly represent the situation in the human body.

Triple-negative breast cancer (TNBC) constitutes a small subtype (~15%) of breast cancer, but causes the majority of breast cancer-related deaths.

Invasive lobular carcinoma (ILC) is the second most common type of breast cancer.

Promotors: C.A.J. Knibbe, Anthonius de Boer, Co-promotor: M.M.J. van der Vorst

Promotor: G.F.A. Kersten, W. Jiskoot, Co-promotor: B. Metz

De Faculteit der Wiskunde en Natuurwetenschappen is gevestigd op verschillende locaties in het Bio Science Park Leiden.

Promotores: C.A.J. Knibbe; J.N. van den Anker, Co-promotores: H.P.A. van Dongen; B. van Ramshorst

This thesis investigates a pharmacological challenge model induced by Toll-like receptor 7 (TLR7) activation, focusing on skin inflammation and its implications for early drug development.

Inflammatory skin diseases such as psoriasis and atopic dermatitis can severely impact both the physical and social aspect of a patient’s life. Aberrant inflammatory responses are thought to be a major causative factor in these disorders, but an abundance of different factors contribute to the development…

The investigation into the bidirectional communication between the gut and its host is a rapidly growing area of research, attracting considerable attention for its potential to provide crucial insights into health and disease.

Drug induced organ toxicity is the main problem of the drug development and drug usage in the clinic. The liver and kidneys are the most sensitive organs towards drug induced toxicity.

This thesis focuses on enhancing predictions of central nervous system drug exposure using the LeiCNS-PK3.0, a physiologically based pharmacokinetic model.

According to the amyloid cascade hypothesis, accumulation of beta-amyloid (Aβ) peptides initiates the pathological cascade in Alzheimer's disease (AD).

This thesis investigated miniaturized mass spectrometry (MS)-based analytical methods to address challenges in analyzing biomass-restricted biological samples.

Nieuw handboek geschiedfilosofie van Herman Paul in de reeks 'Wetenschapsfilosofie in context'.

M. Danhof, Co-promotores: J. Freijer, A. Yassen

Particles are omnipresent in biopharmaceutical products. In protein-based therapeutics such particles are generally associated with impurities, either derived from the drug product itself (e.g. protein aggregates), or from extrinsic contaminations (e.g. cellulose fibers).

Atherosclerosis is the most prominent underlying pathology of cardiovascular disease and an important cause of major adverse cardiovascular events.

Waarom spraken negentiende-eeuwse wetenschappers zo graag over toewijding, vlijt en ijver? Waarom wijdde Robert Fruin zijn oratie aan onpartijdigheid en riep Abraham Kuenen zijn studenten op tot waarheidsliefde?

Promotor: C.A.J. Knibbe, Co-promotores: H.P.A. van Dongen, B. van Ramshorst

This thesis focuses on using liposomes in two different treatment strategies; vaccination (or immunotherapy) and delivery of a small molecule, and in two different disease models; cancer and atherosclerosis.

Triple-negative breast cancer (TNBC) is a subtype of breast cancer characterized by limited treatment options and unfavorable clinical outcomes. Therefore, the research described in this thesis focused on the exploration of novel targeted therapies for TNBC.

Promotores: Prof.dr. J. Kuiper, Prof.dr. P.H.A. Quax, Co-promotor: I. Bot

Promotor: Prof.dr. B. van de Water

Solute carrier (SLC) transporters are a large and diverse class of relatively understudied transmembrane proteins.

The aim of this thesis is to expedite and ensure the systematic accuracy of clearance scaling from adults to paediatric patients, with a special focus on drugs undergoing hepatic metabolism.

An interdisciplinary collection of the best international scholarship on Muslim youth.

Promotor: Prof.dr. B. van de Water, Co-promotores: L.T.M. van der Ven, A.S. Kienhuis

Promotor: M. Danhof, Co-promotor: E.C.M. de Lange

Utilizing the polymeric platform of polypept(o)ides, this thesis describes synthesis and investigation of novel triblock copolymers to obtain carrier systems with multiple compartments for efficient siRNA delivery. Although the individual microstructure of nanoparticles differs depending on the polymeric…

Promotores: J. Jonkers, K.E. de Visser

Atherosclerosis is the most important underlying process that drives cardiovascular disease, and is characterized by an accumulation of cholesterol which triggers an inflammatory response in the vessel wall.

Parkinson's disease is the second most common neurodegenerative disease in the world. One of its symptoms is the loss of dopaminergic neurons in the substantia nigra pars compacta.

Acute cardiovascular clinical events such as myocardial infarction and cerebral stroke represent the major cause of death in Western societies. These pathologies are primarily resulting from atherosclerosis, a progressive condition characterized by the accumulation of lipids, immune cells, and fibrous…

Antimicrobial drugs constitute a fundamental part of modern medicine. The global rise in antimicrobial resistance poses a major threat to global health.

Promotor: B. van de Water

Prematurely born neonates require, amongst others, pharmaceutical therapy. Dosing guidelines for these therapies are often based on data from term born neonates or older infants, while these are not necessarily similar to prematurely born neonates.

There is an urgent need for more physiologically relevant cell culture methods to guide compound selection in pre-clinical stages of the drug development pipeline.

Promotor: Prof.dr. B. van de Water

Promotor: T. Hankemeier, Co-promotor: T.H. Reijmers

Promotores: T. Hankemeier; R. Berger, Co-promotor: R.J. Vreeken

The lipids in the uppermost layer of the skin, the stratum corneum (SC), play an important role in the skin barrier function. The three main SC lipid classes are ceramides (CER), cholesterol and free fatty acids.

De juiste student op de juiste plek

Promotor: Prof.dr. T. Hankemeier, Co-promotor: Theo Reijmers

In this thesis, the role of mast cells in atherosclerosis and novel therapeutic strategies to inhibit atherosclerosis progression are discussed. The first part of the thesis specifically focuses on the relation between mast cells and advanced human atherosclerotic plaque characteristics.