The spindle-assembly checkpoint (SAC) is a safety mechanism which secures accurate chromosome segregation during mitosis.

PNEC-pro is a user-friendly screening tool for professionals dealing with the assessment of surface water quality. The tool calculates local, watertype specific no-effect concentrations (PNEC) of copper, lead, nickel and zinc based on biotic ligand models (BLMs).

Several single-stranded RNA viruses make use of Xrn1-resistant RNAs in their 3’ untranslated regions of their genome RNAs in order to increase their pathogenicity.

Biopolymer sequencing with graphene edge-based tunnel junctions has the potential to overcome current limitations with the third generation of sequencing based on biological nanopores.

Promotor: Prof.dr. G.R. de Snoo, Co-promotor: R. Heijungs

Viral hemorrhagic fever (VHF) is a group of acute diseases caused by highly infectious viruses including Ebola, Lassa, Dengue viruses. Its high mortality rate poses high risk to public health, however, studies on VHF have been hampered due to the non-availability of proper models and incomplete knowledge…

Wij richten ons op natuurlijke producten van onderzoek, de traditionele geneeskunde, etnobotanie en fytochemie.

Promotor: Prof.dr. H.P. Spaink, Co-promotor: Dr. M. van der Zee

This thesis describes the design and synthesis of fragments of various cell wall carbohydrates of the Streptococcus species, including the branched Group B-specific antigen (GBC) of Group B Streptococcus, glycerol phosphate (GroP) modified group A carbohydrate (GAC), and the O-acetylated type 1 capsular…

Promotor: Prof.dr. J.G.E.M. Fraaije, Co-promotor: A. Kros

This thesis addresses the challenge of sustainable urban development by analyzing the interplay between green and grey infrastructure to optimize resource use and ecosystem services.

The mycorrhizal symbiosis is among the most widespread species interactions on Earth⁠. This thesis focuses on orchid mycorrhiza, a unique mycorrhizal type that has caught scientists’ attention for centuries⁠.

Na het afronden van de bacheloropleiding volgen verreweg de meeste studenten de masteropleiding. De Universiteit Leiden stimuleert studenten om na hun bacheloropleiding door te gaan met de masteropleiding.

Promotor: H.S. Overkleeft, Co-promotor: M. van der Stelt

Promotor: T. Schmidt, Co-Promotor: B.E. Snaar-Jagalska

Promotor: Prof.dr. W.J.G.M. Peijnenburg

Plant diversity is essential for us and our planet as it sustains the stability of our ecosystems, provides vital materials and food to us and supports many ecosystem services.

Promotor: Prof.dr. J.P. Abrahams, Prof.dr. M. van Heel

Promotor: Jaap Brouwer, Co-promotor: Remus Th. Dame

Mycoheterotrophy is a particular mode of life in which plants obtain carbohydrates from their associated fungal partners, instead of by using photosynthesis.

This Thesis focuses on the design and synthesis of ADP-ribosylated compounds that can be applied in biological studies.

Promotor: G.R. de Snoo, Co-promotor: C.J.M. Muster

Inverse problems are problems where we want to estimate the values of certain parameters of a system given observations of the system. Such problems occur in several areas of science and engineering. Inverse problems are often ill-posed, which means that the observations of the system do not uniquely…

Promotores: Prof.dr. G.A. van der Marel, M. van Heel

The rapidly increasing commercial application of metallic nanoparticles within products will inevitably enhance the amount of NPs being released into soil.

Promotores: H.A. Udo de Haes, H.H.T. Prins, Co-promotor: H.H. de Iongh

Promotores: Prof. dr. G.R. (Geert) de Snoo, Dr. E. (Ester) van der Voet

In a typical glycosylation reaction, a donor is activated to form a (variety of) electrophilic species which can react with a nucleophilic acceptor, following a reaction mechanism having both SN1 and SN2 character.

The endocannabinoid receptors CB1R and CB2R are involved in a plethora of processes, and consequently are involved in many pathological conditions. Their wide distribution makes the CBRs both an interesting therapeutic target and hard to study.

This thesis describes the use of bioorthogonal proteins in immunological settings. It provides an introduction towards the field of protein modification, which was used throughout the thesis for the expression of proteins containing unnatural amino acids.

Cardiovascular diseases (CVDs) are the leading global cause of mortality. Endothelial dysfunction, an early reversible stage of CVD development, is marked by decreased nitric oxide production linked to Ca2+ influx in endothelial cells.

This ecological community has long been known to affect host biology, and their diverse roles have been further clarified in recent years following numerous studies of animal:microbiota interactions in diverse systems.

Diacylglycerol lipases (DAGLα and DAGLβ) are responsible for the biosynthesis of the endocannabinoid 2-arachidonoylglycerol (2-AG) in the brain and peripheral tissues. Selective DAGLβ inhibitors have been proposed as a potential treatment for inflammatory diseases with reduced potential for central…

Trigonostemon Blume is a plant genus in the family Euphorbiaceae comprising 59 species.

Vitamin A or retinol is essential in embryonic development, the visual cycle and the immune system.

The study of orchid flowers, fruits, and inflorescences is crucial due to the remarkable diversity of orchid species and their unique adaptations to pollinators and seed dispersers. However, our understanding of the evolution and development of these organs within the orchid family remains limited.

Despite the fact that gold nanoparticles (GNPs) are one of the most studied nanoparticles, there is still a necessity for new approaches allowing for effective protective coating to enable wider use of GNPs in biomedical applications.

CML rapport 192

Plastics have become an integral part of modern society due to their versatility and durability.

Natural resources often stretch across borders that separate modern nation states. This can create conflict and limit opportunities for regulated consumption of their goods and services, but also provide opportunities for joint multinational efforts that exceed single country capabilities.

The specialisation Science Communication and Society (SCS) concerns science communication in a broad sense. The program focuses on the link between science and society and covers knowledge and skills in the field of science communication.

Pharmaceutical companies, governments and the general public have become increasingly aware that animal models used in drug testing lack vital aspects to serve as an accurate representation of human biology. As models of the human body should become more physiologically relevant, animal models no longer…

Nucleoid associated proteins maintain the architecture of the bacterial chromosome and regulate gene expression, hinting that their role as transcription factors may involve local three-dimensional chromosome re-modelling.

Advanced UHF MRI techniques provide powerful tools for studying the zebrafish brain, an emerging model in neurological research. In this work, various MRI and localized MRS methods are optimized and employed for UHF strength for studying the zebrafish brain.

Chemical-induced organ toxicity is a major concern in the development and use of chemicals, including drugs, pesticides, industrial chemicals and cosmetics. In the last decades, the onset and progression of chemical-induced organ toxicity has been linked amongst others to perturbation of mitochondri…

Principles of Environmental Sciences provides a comprehensive picture of the principles, concepts and methods that are applicable to problems originating from the interaction between the living and non-living environment and mankind. Both the analysis of such problems and the way solutions to environmental…

β-Lactamases are enzymes that can break down β-lactam substrates, such as antibiotics, preventing the use of these antibiotics for the treatment of various infectious diseases. However, some compounds, β-lactamase inhibitors, can block these enzymes allowing for possible treatments using a combination…

Promotores: E. van Dijk, G.R. de Snoo. Co-promotor: H. Staats

Lysosomal acid glucosylceramidase (GBA1) is a lysosomal enzyme that degrades glucolipids with its main substrate being glucosylceramide (GlcCer). Defects in the GBA1 gene lead to glycosphingolipidosis Gaucher disease (GD), in which the hydrolysis of GlcCer is impaired and therefore, it accumulates in…

This thesis focuses on the social dimensions of crane and wetland conservation in rural landscapes in Kenya, Uganda and Zimbabwe.