This thesis explores different avenues to develop insurmountable antagonists for CC Chemokine Receptors, such as CCR1, CCR2 and CCR5.

The main subject of this thesis is the design and synthesis of paramagnetic molecules for protein studies with NMR and EPR spectroscopy.

In this Ph.D. thesis, we characterized two novel upstream regulators of JUB1, HB40, and HB22, in Arabidopsis thaliana. In Chapter 2, we described functions of HB40 in modulating growth and development via the regulation of JUB1 and GA inactivation genes GA2OXs.

The following report discusses a survey on the topic of the Sustainable Development Goals that was distributed among researchers at Leiden University in the month of November 2021.

Promotor: Prof.dr. R. Verpoorte, Co-Promotor: Dr. Young Hae Choi

Solute carrier (SLC) transporters are a large and diverse class of relatively understudied transmembrane proteins.

There is an urgent need for more physiologically relevant cell culture methods to guide compound selection in pre-clinical stages of the drug development pipeline.

Optimization tasks in practice have multifaceted challenges as they are often black box, subject to multiple equality and inequality constraints and expensive to evaluate.

This thesis describes the functional analysis of the Arabidopsis AHL15 gene.

This PhD-thesis presents a study on micron-sized particles, so-called colloids. By controlling the chemical and physical properties of these particles, such as the interparticle interaction and the particles’ shape, colloids can act as building blocks that self-assembly into larger structures.

Drug-induced liver injury (DILI) is one of the main reasons for drug attrition during pre-clinical and clinical phases of drug development as well as for drug withdrawal post-marketing.

The way organisms develop from the initial single-cellular state to a complex final assembly like the human body, and how the final body is maintained throughout life, is one of the greatest mysteries and it’s understanding one of the biggest scientific challenges.

This thesis describes the development of improved formulations and alternative delivery strategies for polio vaccination.

Sorghum (Sorghum bicolor) is a major cereal crop that millions of subsistence farmers in Sub-Saharan Africa (SSA) rely on. However, the scourging impact of the root parasitic weed Striga (Striga hermonthica, a.k.a. “witch-weed”) has remained a major constraint hampering the productivity of sorghum in…

De programmagroep Leer- en Gedragsproblemen in het Onderwijs is verantwoordelijk voor onderwijs op het gebied van leerproblemen binnen de bachelor- en masteropleidingen van het Instituut Pedagogische Wetenschappen.

Antibiotic resistance, caused by widespread use of antibiotics, leads to bacterial infections that are difficult, if not impossible, to treat and is a major worldwide health concern.

The function of TLRs in innate immunity has aroused worldwide attention soon after its discovery. Because of the broad functions of TLR2 in innate immunity, the drive for the development of TLR2-targeted vaccines or therapeutic treatments has accelerated in the last decades.

De keten die patiënten voorziet van nieuwe medicijnen, bestaat uit diverse complexe schakels: van fundamentele wetenschap, tot klinische tests, tot productie. Deze hele keten is aanwezig in Leiden. De Universiteit Leiden, het Leids Universitair Medisch Centrum (LUMC) en de bedrijven op het Leiden Bio…

Eveline Crone is lang niet de enige professor puberbrein op het internationale FLUX-congres. Leiden was vier dagen lang het centrum van onderzoek naar hersenontwikkeling. Opvoeders en beleidsmakers ontmoetten wetenschappers tijdens het publiekssymposium op 18 september.

Wij zijn gevestigd in het Pieter de la Courtgebouw van de Faculteit der Sociale Wetenschappen van de Universiteit Leiden.

De masterspecialisatie Sociology of Policy in Practice biedt de mogelijkheid om een onderzoeksstage te doen om het beleid van een bedrijf of organisatie in de praktijk te bestuderen..

In de masterspecialisatie Visual Ethnography leer je de kneepjes van het vak om etnografisch onderzoek met audiovisuele methodes te doen.

The Minor Computational Approaches to Disease, Signaling and Drug Targets (CADSDT) is focused on fundamental scientific research required for discovery of new drug targets and development of new drugs.

What is the optimal design of sandy solutions for sustainable climate change adaption, from an ecological, biophysical and societal point of view?

De eer was aan Professor Simon Portegies Zwart en doctor Jeroen Bédorf om voor het blad IEEE Software te schrijven.

Op 15 September 2015 ontvangt Merlijn van Weerd, coördinator van de Field Course Filippijnen, de prestigeuze Parker/Gentry Award uitgereikt door het Field Museum of Natural History in Chicago. Van Weerd is de eerste Europeaan die deze prijs voor natuuronderzoek en -bescherming ontvangt.

De Nobelprijs voor Scheikunde 2017 is uitgereikt aan Jacques Dubochet, Richard Henderson en Joachim Frank, voor hun ontwikkeling van de cryo-elektronenmicroscopie. Met deze techniek kunnen biomoleculen in 3D in beeld gebracht worden, legt hoogleraar Ultrastructuurbiologie Ariane Briegel uit.

Promotor: H.S. Overkleeft, Co-promotor: M. van der Stelt

Biodiversity enables ecosystems to thrive through the synergy of functional differences among organisms. While human well-being strongly depends on biodiversity-driven ecosystem services, human actions are also at the root of current unprecedented biodiversity declines.

Overkleeft

Cytotoxic T-cells (CTLs) are involved in the clearance of viruses and killing of tumor cells.

Promotores: W.J.G.M. Peijnenburg, G.R. de Snoo, Co-promotor: M.G. Vijver

Dit proefschrift beschrijft een collectie aan alternatieve strategieën voor het begrijpen, ontwerpen en toepassen van lipide nanosystemen, waarin de rol van de bio-nano interacties centraal staan. In het bijzonder wordt gekeken naar de interactie van RNA-lipide nanosystemen, bekend van de toepassing…

Promotores: Prof.dr. H.S. Overkleeft, Prof.dr. G.A. van der Marel

This thesis focuses on the design and synthesis of activity-based probes and inhibitors targeting enzymes involved in the degradation of specific plant glycans.

This thesis explores squaramide-based supramolecular polymers and hydrogels, enhancing their mechanical strength for 3D cell culture.

The primary goal of this research is to deepen our understanding of substrate-enzyme interactions in Glycoside hydrolases. For this purpose, Bacillus circulans xylanase (BcX) has been selected as a well-established model to examine the Michaelis complex and glycosyl-enzyme adduct.

This dissertation contains four works during my PhD. Different biomaterials have been designed based on coiled-coil peptides. These biomaterials have a range of applications, inclusing drug delivery, cell sorting to cell-cell fusion.

Promotor: Prof.dr. M. Ubbink

This doctoral thesis is an effort to understand how lipid phase-separation induced by diacylglycerol analogues in lipid-based nanoparticles affects their in vivo behavior, leading to specific nanoparticle-protein communications and selective cell targeting.

N-acylethanolamines (NAEs), including N-arachidonoylethanolamine (anandamide, AEA), N-palmitoylethanolamine (PEA) and N-oleoylethanolamine (OEA), are signaling lipids that play an important role in different physiological process.

Anthropogenic change is reshaping ecological systems, raising concerns about increasing transmission of infectious diseases. Among these, mosquito-borne diseases may be particularly sensitive to environmental change, as their transmission depends on vector populations.

The main goal of this thesis is to achieve advanced control of binding properties in bis-pyrrolic and macrocyclic pyrrolic receptors by light.

Inaugurale rede

The thesis describes terrestrial field trials aimed to detect side-effects of pesticides within the framework of pesticide registration procedures. Field trials were developed using vascular plants Brassica napus and Poa annua, caterpillars Pieris brassicae and effects on decomposition were studied…

This thesis describes the use of an activity-based proteomics method to study the endocannabinoid system.

Endocannabinoids are lipid messengers that modulate various physiological functions by activating the cannabinoid CB1 and CB2 receptors. While the metabolism of endocannabinoids and their action at the CB receptors is fairly well studied, the molecular mechanisms governing their release and transport…