This thesis describes the synthesis and biochemical evaluation of a variety of cyclophellitol based activity-based probes and inhibitors targeting various endo- and exo-acting retaining glycosidases. In the last two decades a variety of probes and inhibitors for (hemi)cellulose degrading enzymes have…

This thesis investigates the relations between metabolism and anatomy through the use of Magnetic Resonance Imaging (MRI).

This thesis investigates the evolution and fate of gaseous exoplanets, which are continuously shaped by stellar activity across both short and long timescales.

Wetenschappers van de Universiteit Leiden hebben een doorbraak bereikt in de bestudering van de erfelijke stofwisselingsziekte van Pompe. Samen met hun Yorkse collega’s ontwierpen zij een molecuul dat zich bindt aan het enzym dat aan de basis staat van het ziekteverloop. De bevindingen zijn gepubliceerd…

The adenosine receptors are proteins that reside in the extracellular membranes of cells. Activation of adenosine receptors plays a role in many physiological and pathological processes, such as immune responses and cancers.

This thesis describes the development of a variety of mannosylated conjugates. Antigen presenting cells bear mannoside recognizing receptors that actively transport antigen into the cell. This thesis exploits this feature for the development of improved vaccines.

Multicopper oxidase, laccase, can efficiently reduce oxygen to water and are mostly used in the enzymatic biofuel cells.

By growing in a soil plants change the biotic and abiotic properties of the soil in which they grow.

This thesis describes the use of bioorthogonal proteins in immunological settings. It provides an introduction towards the field of protein modification, which was used throughout the thesis for the expression of proteins containing unnatural amino acids.

This dissertation was to develop effective graphene-based nanofluidic devices by developing scalable fabrication methods and elucidating proton transport mechanisms.

Promotor: J. Schaye

Promotor: B. van de Water

In the last decades, activity-based protein profiling (ABPP) has emerged as a powerful chemical tool that may aid the ever-challenging drug discovery process.

This dissertation combines the use of defined microenvironments, high-resolution fluorescence microscopy, and time-resolved analysis, to study intracellular and cellular motility.

Humanity stands at a pivotal juncture, facing the dual challenge of environmental sustainability and rapid population growth, projected to exceed 11 billion by century’s end.

The first steps are made in personalised cancer vaccination strategies, which aim to induce a more specific immune response with fewer side effects. The activation of cytotoxic T cells is crucial for an effective immune response.

Biopolymer sequencing with graphene edge-based tunnel junctions has the potential to overcome current limitations with the third generation of sequencing based on biological nanopores.

Marine sponges are important members of reef ecosystems, as they play ecological roles that are essential for the health of the reef.

Promotor: H.V.J. Linnartz

This thesis provides an overview of the development of the Pair Configuration to Molecular Activity Coefficient (PAC-MAC) model.

This thesis focuses on the design and synthesis of activity-based probes and inhibitors targeting enzymes involved in the degradation of specific plant glycans.

Promotor: A.P.IJzerman Co-promotor: A. Bender

BlaC is the β-lactamase of Mycobacterium tuberculosis. We show that it can recover from inhibition by clavulanic acid and that phosphate helps it do so.

This thesis focuses on developing wafer-scale two-dimensional (2D) materials by combining synthesis, transfer, characterization, and device integration of graphene, hexagonal boron nitride (h-BN), and BN-doped amorphous carbon.

This Thesis shows discoveries in non-linear astrochemical kinetics as well as a deeper analysis of dark clouds chemistry.

Multiple stars, that is two or more stars composing a gravitationally bound system, are common in the universe.

Vitamin A or retinol is essential in embryonic development, the visual cycle and the immune system.

This thesis focuses on protoplanetary disks: flattened structures of gas and dust around young stars in which planets are expected to form and grow.

Streptomyces bacteria are a valuable source of natural products, many of which are used in the clinic or in biotechnology.

Na het afronden van de bacheloropleiding volgen verreweg de meeste studenten de masteropleiding. De Universiteit Leiden stimuleert studenten om na hun bacheloropleiding door te gaan met de masteropleiding.

To address the fundamental questions of how life on Earth emerged and how common life may be in the Universe, it is crucial to know the chemical composition of the planet-forming material.

This thesis advances our understanding of lipid uptake, a vital first step in lipid metabolism, by developing innovative click chemistry-based tools to study how immune cells internalize lipids.

This thesis describes the use of an activity-based proteomics method to study the endocannabinoid system.

Cytotoxic T-cells (CTLs) are involved in the clearance of viruses and killing of tumor cells.

Promotor: Prof.dr. H.L.V. Linnartz

A new type of tripodal squaramide-based supramolecular hydrogels is developed and studied.

In this thesis an activity-based probe was discovered that could visualize the activity of PLAATs. With an optimized gel-based ABPP assay in hand, screening of a compound library led to the discovery of alpha-ketoamides as a hit for PLAAT3.

Breeding for resistance becomes more and more important because we want to reduce the use of pesticides. A fast and cheap alternative can be to make use of morphological or chemical markers.

Promotie

As a contribution to the changing legislation and evolving societal attitudes concerning environmental issues, this project aims to enhance and manipulate the plants’ own natural defense mechanisms against western flower thrips (WFT).

The human body consists of hundreds, perhaps thousands of different types of cells, each with different morphologies and functions, despite having the same genome.

Electrocatalysis allows for storing electricity or converting it into chemical bonds, producing chemical building blocks and fuels using renewable resources.

Promotores: Prof.dr. G.A. van der Marel, M. van Heel

Organic molecules in interstellar space are important as they influence the structure of galaxies and star formations. Studying catalytic processes in space allows us to understand how molecular species are formed and chemically evolved in the interstellar medium and solar system objects.

Promotor: Prof.dr. T. Hankemeier, Co-promotor: Theo Reijmers

This PhD-thesis presents a study on micron-sized particles, so-called colloids. By controlling the chemical and physical properties of these particles, such as the interparticle interaction and the particles’ shape, colloids can act as building blocks that self-assembly into larger structures.

This thesis discusses the physical and chemical processes than influence the composition of forming planets.