Neuraminidases are enzymes that cleave glycosidic linkages of sialic acid. These enzymes are involved in influenza infections as well as in many cellular processes in mammals and micro-organisms.

The work described in this Thesis is focused on the assembly of oligosaccharide fragments derived from a fungal polysaccharide, galactosaminogalactan (GAG) and fragments of the exopolysaccharide Pel, generated by Pseudomonas aeruginosa.

Evolution acts via mutations in amino acid sequences. Substitution of essential amino acids leads to a nonfunctional protein. l.

Projecten van de afdeling Environmental Biology

Cyclic peptides are investigated as a platform to induce different orientations between various ligands.

DNAmarkerpoint heeft als doel ecologie, evolutie en biodiversiteit beter te begrijpen door het bestuderen van modern en ancient DNA.

Promotor: Prof.dr. M. Ubbink, Co-promotor: G. Siegal

Nicotinamide adenine dinucleotide (NAD+) is the substrate used for the introduction of the ubiquitous and highly dynamic PTM in which either one or multiple adenosine diphosphate ribose (ADPr) moieties are covalently attached to a nucleophilic side chain of an specific amino acid in the target protein…

What is the optimal design of sandy solutions for sustainable climate change adaption, from an ecological, biophysical and societal point of view?

De Nobelprijs voor Scheikunde 2017 is uitgereikt aan Jacques Dubochet, Richard Henderson en Joachim Frank, voor hun ontwikkeling van de cryo-elektronenmicroscopie. Met deze techniek kunnen biomoleculen in 3D in beeld gebracht worden, legt hoogleraar Ultrastructuurbiologie Ariane Briegel uit.

This thesis focuses on the design and synthesis of activity-based probes and inhibitors targeting enzymes involved in the degradation of specific plant glycans.

Overkleeft

Biodiversity enables ecosystems to thrive through the synergy of functional differences among organisms. While human well-being strongly depends on biodiversity-driven ecosystem services, human actions are also at the root of current unprecedented biodiversity declines.

Promotores: W.J.G.M. Peijnenburg, G.R. de Snoo, Co-promotor: M.G. Vijver

Dit proefschrift beschrijft een collectie aan alternatieve strategieën voor het begrijpen, ontwerpen en toepassen van lipide nanosystemen, waarin de rol van de bio-nano interacties centraal staan. In het bijzonder wordt gekeken naar de interactie van RNA-lipide nanosystemen, bekend van de toepassing…

hotocatalysis has emerged as a powerful tool for enabling challenging transformations in organic synthesis. This thesis explores the development of several photocatalytic reactions designed to form carbon-heteroatom bonds from less-functionalized precursors.

Cytotoxic T-cells (CTLs) are involved in the clearance of viruses and killing of tumor cells.

Promotor: H.S. Overkleeft, Co-promotor: M. van der Stelt

The research presented in this thesis explores the chemotherapeutic potential of metal-based compounds as chemotherapy agents, with an initial focus on the synthesis and DNA interaction studies of platinum and palladium compounds utilizing the [Pt(bapbpy)]2+ scaffold. The study identifies intercalation…

Promotores: Prof.dr. W.J.G.M. Peijnenburg & Dr. M.G. Vijver

Anthropogenic change is reshaping ecological systems, raising concerns about increasing transmission of infectious diseases. Among these, mosquito-borne diseases may be particularly sensitive to environmental change, as their transmission depends on vector populations.

This thesis combines field and laboratory studies to address some of the most pressing questions in the field of microplastic research.

The research described in this thesis combines the latest insights in lysosomal function with lysosome centred cell signalling.

Endocannabinoids are lipid messengers that modulate various physiological functions by activating the cannabinoid CB1 and CB2 receptors. While the metabolism of endocannabinoids and their action at the CB receptors is fairly well studied, the molecular mechanisms governing their release and transport…

Carbohydrates are present on the surface of bacteria making them suitable antigen candidates for vaccine development. This thesis deals with the synthesis of two carbohydrate-based components; the capsular polisaccharide of S. aureus type 5 and teichoic acids from staphilococci and enterococci speci…

Soil biodiversity is of great importance for the maintenance of multifunctionality in terrestrial ecosystems.

Promotores: Prof.dr. H.S. Overkleeft, Prof.dr. G.A. van der Marel

Promotor: G.R. de Snoo, Co-promotores: M.G. Vijver, J.P.M. Vink

Promotor: Prof.dr. M. Ubbink

This thesis explores squaramide-based supramolecular polymers and hydrogels, enhancing their mechanical strength for 3D cell culture.

The thesis describes terrestrial field trials aimed to detect side-effects of pesticides within the framework of pesticide registration procedures. Field trials were developed using vascular plants Brassica napus and Poa annua, caterpillars Pieris brassicae and effects on decomposition were studied…

Inaugurale rede

This thesis describes the use of an activity-based proteomics method to study the endocannabinoid system.

This dissertation contains four works during my PhD. Different biomaterials have been designed based on coiled-coil peptides. These biomaterials have a range of applications, inclusing drug delivery, cell sorting to cell-cell fusion.

The primary goal of this research is to deepen our understanding of substrate-enzyme interactions in Glycoside hydrolases. For this purpose, Bacillus circulans xylanase (BcX) has been selected as a well-established model to examine the Michaelis complex and glycosyl-enzyme adduct.

Promotor: Prof.dr. G.A. van der Marel, Co-Promotor: J.D.C. Codée

The main goal of this thesis is to achieve advanced control of binding properties in bis-pyrrolic and macrocyclic pyrrolic receptors by light.

This thesis focused on the exploration of monolayer graphene as a membrane in direct methanol fuel cell (DMFC) applications, with improvements achieved through plasma exposure or damage- free chemical functionalization.

Promotor: Prof.dr. J.P. Abrahams

This thesis describes the antimicrobial discovery strategy developed in our group, the den Hertog Group at the Hubrecht Institute. It includes a cultivation-based screening approach for novel antimicrobial agents from the source of fungi, and a bacterial time-lapse imaging approach for antimicrobial…

This multidisciplinary minor provides students with knowledge on how and why humans became the way they are. The minor focuses on the evolution of the species Homo sapiens from other hominin lineages and animal ancestors, and on the various factors shaping this process. Not only is human evolution of…

If mankind wants to survive into the 22nd century, we will need to change the way we live drastically. The human impact on our planet is everywhere, we are changing the atmosphere, the oceans and the land, leading to global warming, climate change, loss of biodiversity and pervasive pollution. There…

Promotor: G. A. van der Marel, Co-promotor: D. V. Filippov

Leiden University offers a unique range of summer courses where you can broaden or deepen your knowledge or share your expertise with fellow students. The choice of academic courses is very diverse, ranging from Law, Big Data, Languages and Linguistics to Maths and Natural Sciences, Biology, Biomedical…

N-acylethanolamines (NAEs), including N-arachidonoylethanolamine (anandamide, AEA), N-palmitoylethanolamine (PEA) and N-oleoylethanolamine (OEA), are signaling lipids that play an important role in different physiological process.

This doctoral thesis is an effort to understand how lipid phase-separation induced by diacylglycerol analogues in lipid-based nanoparticles affects their in vivo behavior, leading to specific nanoparticle-protein communications and selective cell targeting.