The research described in this thesis focused on the use of bioorthogonal antigens to investigate immunological processes in antigen presenting cells. Bioorthogonal antigens are antigenic proteins produced through recombinant expression in a methionine auxotrophic E. coli strain.

Microscopic insight on lipid-based nanomedicine in vivo remains limited to the perception of the knowledge that could be obtained: if we interpret only what we see, then we only believe to know. Although believing to know encapsulates an undefined amount of uncertainty in the exploration of lipid-based…

This dissertation explores new chemical strategies for the synthesis of self-adjuvanting peptide conjugates and immunomodulatory ligands aimed at targeted activation of the immune system.

A key step in the synthesis of oligosaccharides and glycoconjugates is the formation of the glycosidic bond.

Veiligheidsvraagstukken staan bovenaan het lijstje van de meest dringende problemen van de huidige samenleving.

Synthetic methodlogy is described, aiding in the synthetic preparation of putative inhibitors of retaining and inverting glycosidases and glycosyl transferases. All constructs are cyclophellitol-based cyclitols.

The thesis describes the development, synthesis and biological evaluation of several proteasome inhibitor designs. Mainly, this work focusses on designing proteasome inhibitors that selectively inhibit the immunoproteasome, while leaving the constitutive proteasome mostly, if not completely, untouch…

Design and synthesis of two 3-substituted swainsonine derivatives with the aim to improve the potency and selectivity towards Golgi alpha-mannosidase II.

The chemical synthesis of inhibitors and probes targeting endo-glycosidases.

This thesis describes the design and synthesis of novel small molecules based on sulfur-containing heterocycles for the inhibition of glycosidases and glycosyltransferases related to Fabry and Pompe disease.

The aim of the research described in this thesis was to use structure-based design to optimise multiple properties of aryl sulfoxide monoacylglycerol lipase inhibitors as anti-inflammatory analgesics.

Hoe dragen vetten en enzymen in de hersenen bij aan multiple sclerose? In zijn promotieonderzoek combineerde Daan van der Vliet chemie en neurowetenschap om nieuwe inzichten te krijgen in het ontstaan van hersenziekten.

Jelle van Buuren, universitair docent bij het Institute of Security and Global Affairs, bespreekt bij One World de reden dat rechtsextremisten zich ook in de coronasceptische beweging bevinden.

Bio-Farmaceutische Wetenschappen staat met een overall score van 89.9 op de 9e plaats wereldwijd, de 6e plaats binnen Europa en de 1e plaats in Nederland in de QS World University Rankings, een uitzonderlijke prestatie. Dit blijkt uit de lijst die onderwijsadviesbureau QS publiceerde.

Viral diseases constitute one of the major challenges in modern medicine. Membrane-targeting have broad-spectrum potential and could distinguish viral membranes from the host cell plasma membrane by the difference in membrane curvature.

Within this thesis the central stage is taken by the discovery and investigation of transglycosylation of sterols. First, investigation focuses on the development of a method to accurately detect and quantify glucosylated metabolites in biological materials.

This Thesis describes the synthesis of D-alanylated ribitol-based wall teichoic acids from S. aureus to probe their interaction with immune-binding partners.

This thesis aimed to investigate the impact of exposure dynamics, relative contributions of ENPs(particle) and ENPs(ion), and dosing regimens on the toxicity of ENPs varying in different physico-chemical properties, on the composition and functioning of soil microbial communities.

As a virtually inexhaustible source, solar energy plays a major role in future global energy scenarios.

Synthetic hydrogels that mimic the natural extracellular matrix in the biophysical and biochemical cues it provides to cells are in high demand, however the cell phenotypes as they are observed in vivo in numerous cases have yet to be attained.

In order to be able to develop effective medicine and treatments to prevent or cure autoimmune diseases or cancer we need to understand the mechanisms how they arise and what drives their course.Unravelling the fundamental molecular mechanisms influencing the onset and course of diseases such as allergies,…

The majority (80%) of e-waste generated globally are recycled informally in developing countries.

Promotor: H.S. Overkleeft, J. Neefjes, Co-promotor: M. van der Stelt

Promotor: Prof.dr. J.G.E.M. Fraaije, Co-promotor: A. Kros

Promotor: G.P. van Wezel, Co-promotor: Y.H. Choi

Over several decades, a variety of computational methods for drug discovery have been proposed and applied in practice. With the accumulation of data and the development of machine learning methods, computational drug design methods have gradually shifted to a new paradigm, i.e. deep learning methods…

Promotores: Prof.dr. H.S. Overkleeft, Prof.dr. G.A. van der Marel

Dr. Moritz Jesse, universitair hoofddocent Europees Recht bij het Europa Instituut Leiden, heeft de leden en het publiek van het panel

Op vrijdag 30 september gaan tijdens het debat ‘Human Rights and the World Cup Qatar’ verschillende gasten met een achtergrond op het gebied van mensenrechten, rechten, politiek en internationale betrekkingen met elkaar in gesprek. KNVB-secretaris-generaal en alumnus Gijs de Jong treedt op om meer informatie…

Solliciteer nu voor de LUCIS Summer School on Philology and Manuscripts from the Muslim world. De summer school vindt plaats in Leiden van dinsdag 15 augustus 2017 tot en met vrijdag 25 augustus 2017. Solliciteren kan tot vrijdag 16 juni 2017.

Neuraminidases are enzymes that cleave glycosidic linkages of sialic acid. These enzymes are involved in influenza infections as well as in many cellular processes in mammals and micro-organisms.

Cyclic peptides are investigated as a platform to induce different orientations between various ligands.

Evolution acts via mutations in amino acid sequences. Substitution of essential amino acids leads to a nonfunctional protein. l.

The thesis explores how interstellar chemistry evolves as a function of time and changing physical architectures during the formation of stars.

Nicotinamide adenine dinucleotide (NAD+) is the substrate used for the introduction of the ubiquitous and highly dynamic PTM in which either one or multiple adenosine diphosphate ribose (ADPr) moieties are covalently attached to a nucleophilic side chain of an specific amino acid in the target protein…

Promotor: Prof.dr. M. Ubbink, Co-promotor: G. Siegal

Endocannabinoids are lipid messengers that modulate various physiological functions by activating the cannabinoid CB1 and CB2 receptors. While the metabolism of endocannabinoids and their action at the CB receptors is fairly well studied, the molecular mechanisms governing their release and transport…

This dissertation aims to advance understanding of amoeboid single cell migration under the influence of changing physicochemical environmental properties. The particular focus is on the relatively unknown influence of differing physical properties in the extracellular environment, such as the migratory…

The research described in this thesis combines the latest insights in lysosomal function with lysosome centred cell signalling.

Promotor: G. J. Kroes

We showed that constitutive and inducible chemical and morphological defenses against Western flower thrips differ between tomato and chrysanthemum plants.

This dissertation contains four works during my PhD. Different biomaterials have been designed based on coiled-coil peptides. These biomaterials have a range of applications, inclusing drug delivery, cell sorting to cell-cell fusion.

The primary goal of this research is to deepen our understanding of substrate-enzyme interactions in Glycoside hydrolases. For this purpose, Bacillus circulans xylanase (BcX) has been selected as a well-established model to examine the Michaelis complex and glycosyl-enzyme adduct.

In the past decade many population studies have been performed with the Atacama Large Millimeter/submillimeter Array (ALMA) to understand the bulk properties of protoplanetary disks around young stars. These population studies mostly consisted of low mass stars, with relatively few of the disks around…

The research presented in this thesis explores the chemotherapeutic potential of metal-based compounds as chemotherapy agents, with an initial focus on the synthesis and DNA interaction studies of platinum and palladium compounds utilizing the [Pt(bapbpy)]2+ scaffold. The study identifies intercalation…

Nature uses a special class of histone proteins, histone variants, to modulate the properties of chromatin at defined genomic locations.

This thesis combines field and laboratory studies to address some of the most pressing questions in the field of microplastic research.

Promotors: Prof.dr. E. Bouwman, Prof.dr. E. Drent

Promotor: H.S. Overkleeft, Co-promotor: M. van der Stelt