This thesis comprises the development of reproducible synthesis procedures for two-dimensional materials (composed of boron, nitrogen and carbon) on different metallic surfaces.

Photocatalysis has emerged as a powerful tool for enabling challenging transformations in organic synthesis. This thesis explores the development of several photocatalytic reactions designed to form carbon-heteroatom bonds from less-functionalized precursors.

De Universiteit Leiden is door de Europese Commissie erkend als een instelling met een ijzersterk HR-beleid voor onderzoekers. Ontdek waarom.

Het centrale vraagstuk is hoe jongeren leren die deelnemen aan maatwerktrajecten bij ROC’s en sociale ondernemingen om perspectief te vinden in school en werk, en welke rol de sociale omgeving en begeleidingspraktijken in scholen en bedrijven daarbij spelen.

Het boek behandelt in 17 hoofdstukken vele facetten van de gevolgen van de vergrijzing voor het arbeids- en socialezekerheidsrecht, in de context van veranderende arbeidsverhoudingen. Een dergelijk breed boek over dit actuele onderwerp is tot nu toe nog niet verschenen. Zo wordt niet alleen de positie…

Promotor: H.J.M. de Groot

Promotor: Prof.dr. M. Ubbink

Carbonaceous asteroids preserve a record of early solar system processes, where minerals and organic molecules, like polycyclic aromatic hydrocarbons (PAHs), offer clues to planetary evolution and prebiotic chemistry.

The long-held desire - to link structure directly to function and to explain molecular mechanisms based on basic chemical or physical principles - is finally coming closer, satisfying not only our scientific curiosity but also offering new solutions to the many challenges in the field of health, energy…

Protein–protein complexes are formed via transient states called encounter complexes that greatly influence the formation of the stereospecific complex.

Schistosomiasis is an acute and chronic disease caused by blood dwelling parasitic trematodes of the genus Schistosoma, and it is classified as the second most socioeconomically devastating parasitic disease, second only to malaria.

This thesis describes the use of a combined approach of computational and experimental techniques to gain novel insights to understand the glycosylation reaction and its reactive intermediates.

Promotor: J.G.E.M. Fraaije, Co-Promotor: A. Kros

Phospholipase A/acyltransferase 3 (PLAAT3) and PLAAT4 are enzymes involved in the synthesis of bioactive lipids. Despite sequential and structural similarities, the two enzymes differ in activity and specificity.

Promotor: J.P. Abrahams, Co-promotor: T. Grüne

Every organisms in the tree of life faces the same challenge: the length of its DNA exceeds the volume of the cell it needs to fit in. Several strategies have evolved to solve this problem, one of them being the expression of proteins that bind and organize the DNA.

Parkinson's disease is the second most common neurodegenerative disease in the world. One of its symptoms is the loss of dopaminergic neurons in the substantia nigra pars compacta.

This thesis seeks to combine two different research topics; Multi-Objective Optimization and Complex Network Analysis.

Bicarbonate buffer is largely found in nature due to its ability to regulate pH variations around neutral values. As the pH changes, so does the speciation of the buffer.

Lysosomal acid glucosylceramidase (GBA1) is a lysosomal enzyme that degrades glucolipids with its main substrate being glucosylceramide (GlcCer). Defects in the GBA1 gene lead to glycosphingolipidosis Gaucher disease (GD), in which the hydrolysis of GlcCer is impaired and therefore, it accumulates in…

Large areas of space are filled by molecular clouds that consist of gas and dust grains that are the remnants of dead stars. When these clouds start collapsing, the decreasing temperature and increasing density cause gas particles to start accreting onto dust grain surfaces.

Promotor: Prof.dr. B. van de Water

Promotor: H.S. Overkleeft, Co-promotor: M. van der Stelt

In this thesis, bioorthogonal chemistry is combined with correlative light-electron microscopy to selectively label and study pathogenic intracellular bacteria within the host immune cell.

Diacylglycerol lipases (DAGLα and DAGLβ) are responsible for the biosynthesis of the endocannabinoid 2-arachidonoylglycerol (2-AG) in the brain and peripheral tissues. Selective DAGLβ inhibitors have been proposed as a potential treatment for inflammatory diseases with reduced potential for central…

Curved single crystals provide variable, but well-defined surface structures.

Promotor: M.T.M. Koper, Co-Promotor: L.B.F. Juurlink

A large diversity of exoplanetary systems has been found, but it is still unclear what drives this diversity.

In this thesis, we present new laboratory data of interstellar dust analogues.

Promotor: A. Kros

Despite the fact that gold nanoparticles (GNPs) are one of the most studied nanoparticles, there is still a necessity for new approaches allowing for effective protective coating to enable wider use of GNPs in biomedical applications.

Drug-induced liver injury (DILI) is one of the main reasons for drug attrition during pre-clinical and clinical phases of drug development as well as for drug withdrawal post-marketing.

Inspired by natural photosynthesis, perovskite-based photoelectrochemical (PEC) systems are being developed for artificial photosynthesis, aiming to enhance solar-to-hydrogen conversion for green energy.

In a typical glycosylation reaction, a donor is activated to form a (variety of) electrophilic species which can react with a nucleophilic acceptor, following a reaction mechanism having both SN1 and SN2 character.

Promotor: B. van de Water

The spindle-assembly checkpoint (SAC) is a safety mechanism which secures accurate chromosome segregation during mitosis.

Until the 1990s, the only known planets were those in our Solar System. Three decades later, several thousand exoplanets have been discovered orbiting stars other than the Sun, and substantial efforts have been made to explore these strange new worlds through spectroscopic analyses of their atmosphe…

Monoacylglycerol lipase (MAGL) is the principal enzyme responsible for hydrolysis of the endocannabinoid 2-arachidonoylglycerol (2-AG). MAGL inhibition provides several potential therapeutic opportunities, including anti-nociceptive, anti-inflammatory and anti-cancer activity.

The chemical and structural heterogeneity of toxoid vaccines makes their analysis challenging. However, detailed insights on a molecular level can be obtained by mass spectrometry.

The lysosomal β-glucosidase named glucocerebrosidase (GCase) is a retaining β-glucosidase that hydrolyzes the glycosphingolipid glucosylceramide (GlcCer) to ceramide and glucose at acid pH.

Plant-microbe interaction resulted in different physio/chemical responses by host plant and interacting rhizobacteria.

With the energy transition toward a renewable energy supply and a CO2-neutral economy, electrification of the energy system is rising in importance, which leads to the challenge of long-term storage of renewable electricity.

Promotor: Prof.dr. C.W.J. Beenakker

The research aims to explore the evolutionary adaptability of enzymes and the impact of temperature on protein evolution pathways, using M. tuberculosis β-lactamase BlaC as the object of study. Enzymes inherently embody a delicate balance between activity and stability, and the acquisition of new enzymatic…

The recently introduced nature-originated deep eutectic solvents, so-called natural deep eutectic solvents (NADES) are considered as truly green solvents, which composed of natural ingredients found abundantly in organisms.

This Thesis focuses on the design and synthesis of ADP-ribosylated compounds that can be applied in biological studies.

This thesis examines the link between simple molecules and the underlying structure and chemistry within protoplanetary disks - the birthplaces of planets.

Over the last three decades, the discovery of exoplanets has revealed the boundless variety of worlds beyond our own Solar System⁠. Majority of planetary systems contain short-period planets that are larger than Earth but smaller than Neptune⁠.