Promotor: T. Schmidt

De Microscopy Unit huisvest, onderhoudt en coördineert vrijwel alle microscopieapparatuur van het IBL. De beschikbare apparatuur varieert van conventionele licht- en fluorescentiemicroscopen tot confocale laser- en elektronenmicroscopen. Er is bovendien apparatuur beschikbaar voor histologie, met inbegrip…

Single-Molecule Microscopy (SMM) techniques constitute a group of powerful imaging tools that enable researchers to study the dynamic behavior of individual molecules.

There are many interpretations of quantum mechanics, and ultimately experiments are needed to verify or falsify these interpretations.

This thesis presents insights from our study of various correlated electron systems with a scanning tunneling microscope (STM). In ordinary metals, electron-electron interactions exist, but get substantially screened due to the sheer number of electrons.

Electron microscopy has become an extremely important techniquein a wide variety of elds.

This thesis described the development of novel scanning tunneling microscopy techniques to investigate strongly correlated electronic states in quantum matter.

In this thesis we investigated the ability of two-photon multifocal microscopy for single-molecule microscopy in live cells and organisms.

In surface science there is great effort to move from studying simple, flat model surfaces in vacuum to investigating more complex model catalysts in gas environments (in situ). This thesis gives three examples of such studies using microscopy and spectroscopy.

Het doel van deze faciliteit is om het biomedisch onderzoek te versnellen door toegang te bieden tot geavanceerde licht- en elektronenmicroscopie en door het delen van expertise over monstervoorbereiding, beeldvormingsmodaliteiten, gegevensopslag en beeldanalyse.

Promotor: Prof.dr. J.M. van Ruitenbeek, Prof.dr. R.M. Tromp

In this Ph.D. thesis we study the interaction of low energy electrons with thin materials, namely layered materials (graphene, hexagonal boron nitride, molybdenum disulfide) and organic films. At these low energies the quantum mechanical wavelength of the electron wave function is in the order of a…

In this thesis, we develop a novel technique called Optical Near-field Electron Microscopy (ONEM), which aims to combine the advantages of both optical and electron microscopy: the high resolution of electron microscopy and the low sample damage of optical microscopy.

In this thesis, we describe the latest advances in SQUID-detected Magnetic Resonance Force Microscopy (MRFM).

In this thesis we use Josephson and noise scanning tunneling microscopy for the study of conventional, unconventional (iron-based) and disordered superconductors. On the one hand, Josephson scanning tunneling microscopy allows us to directly visualize the superfluid density with high spatial resolut…

In this thesis, we show how MRFM can usefully contribute to the field of condensed-matter.

The dissertation describes TEM experiments on heterogeneous catalysts.

Promotores: Prof.dr. H.P. Spaink & Prof.dr. P.C.W. Hogendoorn Co-promotor: Dr. M.J.M. Schaaf

Promotores: Prof.dr. C.U. Keller, Prof.dr. H.C. Gerritsen

n this PhD thesis, the recombination of different atomic lattices in stacked 2D materials such as twisted bilayer graphene is studied. Using the different possibilities of Low-Energy Electron Microscopy (LEEM), the domain forming between the two atomic layers with small differences is studied.

Promotor: T.H. Oosterkamp

Hoe is grafeen te controleren?

Natuurkundige Daniël Geelen ontwikkelde tijdens zijn promotie een nieuwe microscoop die laag-energetische elektronen gebruikt. Zulke elektronen zijn minder schadelijk voor biologische en organische materialen. Geelen promoveert op 31 mei.

Hoe supergeleiding werkt is voor onderzoekers allang geen geheim meer. De laatste vijf jaar hebben ze vooral aandacht voor het onverklaarbare gedrag van de elektronen die in het binnenste van supergeleiders zitten. Een internationaal onderzoeksteam met onder meer Leidse natuurkundigen zette in juli…

Promotor: B. van de Water

Promotor: B. van de Water

Voor het eerst hebben natuurkundigen het ‘smelten’ in beeld gebracht van elektronen binnen een speciale soort isolator. Het stelt elektronen in staat om vrij te bewegen en verandert de isolator in een metaal of eventueel zelfs een superconductor. Publicatie in Nature Physics.

Om meer opslagcapaciteit van computergeheugen en snellere computers te kunnen bouwen, willen natuurkundigen weten wat de exacte positie is van elektronen in een elektrisch circuit. Prof. Michel Orrit en collega’s gaan een apparaat bouwen om dat te meten. De Stichting Fundamenteel Onderzoek der Materie…

In this thesis the combinatorial use of bioorthogonal labelling and Electron Microscopy (EM)-based imaging techniques is explored to enable observations of specific molecular targets in their ultrastructural context within the cell.

From surface science towards industrial practice using high-pressure scanning tunneling microscopy.

Molecular materials have been a subject of interest in fundamental research and applications for decades, and have been studied as bulk crystals, (thin) films and as individual molecules, due to the large variety in their properties. This dissertation explores pentacene crystals near the two-dimensional…

Het blad Science publiceert drie artikelen tegelijk over een belangrijke ontdekking in vastestoffysica. Leids natuurkundige Jan Zaanen bespreekt deze ontdekking in dezelfde uitgave van 4 maart.

Microscopic insight on lipid-based nanomedicine in vivo remains limited to the perception of the knowledge that could be obtained: if we interpret only what we see, then we only believe to know. Although believing to know encapsulates an undefined amount of uncertainty in the exploration of lipid-based…

Grafeen en andere laagjesmaterialen vormen samen compleet nieuwe stoffen. Leidse natuurkundigen leggen de basisregels vast voor het ontwerpen van zulke materialen door te meten hoe de lagen in een stapel met elkaar wisselwerken. Publicatie op 29 november in Nature Communications.

This dissertation revolves around the design and implementation of novel instrumentation and related measurement techniques, at the single molecule level, for use in biophysical research. Chapter 1 presents an introduction to the field of fluorescence-based single molecule measurements. In particular,…

Natuurkundigen van de Universiteit Leiden en Stichting FOM hebben een manier gevonden om de eigenschappen van 'slimme', door de mens ontworpen materialen beter te begrijpen. Hun methode onthult hoe op elkaar gestapelde laagjes in zo’n materiaal samenwerken om het geheel tot een hoger niveau te tillen.…

In this thesis, the focus lies on studying glucocorticoid receptor dynamics in living cells with the aim of understanding how this transcription factor finds its DNA target sites to regulate transcription.

This thesis comprises the development of reproducible synthesis procedures for two-dimensional materials (composed of boron, nitrogen and carbon) on different metallic surfaces.

Bacteria have the ability to alter their morphology in order to adapt to changing environments.

Materials with strongly correlated electrons show some of the most mysterious and exotic phases of quantum matter, such as unconventional superconductivity, quantum criticality and strange metal phase.

In this thesis, bioorthogonal chemistry is combined with correlative light-electron microscopy to selectively label and study pathogenic intracellular bacteria within the host immune cell.

Promotor: Prof.dr. J.P. Abrahams, Prof.dr. M. van Heel

This doctoral thesis is an effort to understand how lipid phase-separation induced by diacylglycerol analogues in lipid-based nanoparticles affects their in vivo behavior, leading to specific nanoparticle-protein communications and selective cell targeting.

In condensed matter systems electron-electron interactions, negligible in everyday metals, can dramatically alter the electronic behavior of the system. Examples of such altered behavior include high-temperature superconductivity and modulation of the electron density.

Promotor: Prof.dr. J.W.M. Frenken

The research in this thesis is focused on tomographic reconstruction based on two imaging modalities in electron microscopy.

This thesis focuses on using liposomes in two different treatment strategies; vaccination (or immunotherapy) and delivery of a small molecule, and in two different disease models; cancer and atherosclerosis.

There is an urgent need for more physiologically relevant cell culture methods to guide compound selection in pre-clinical stages of the drug development pipeline.