Kinase inhibitors are key therapeutic agents, particularly in oncology, yet their clinical efficacy is often hampered by off-target effects and limited understanding of their cellular target profiles.

In the emerging field of small-molecule RNA targeting, there is a growing demand for new high-throughput screening methods.

This thesis explored the molecular pharmacological mechanisms of targeting CB2R via investigation of novel drug discovery concepts such as target binding kinetics, allosteric modulation and biased signaling.

In this thesis, we will utilize embryonic zebrafish tumour models to understand the interaction between engrafted human cancer cells and macrophages from the host, test drug administration modalities and anti-cancer efficacies of newly-developed PDT and PACT compounds, and test a light-triggered liposomal…

Bij kanker groeien lichaamscellen ongecontroleerd. Het gedetailleerd in kaart brengen van hoe dit precies gebeurt maakt het mogelijk om efficiënte therapieën te ontwikkelen. Onderzoekers van het Leids Universitair Medisch Centrum (LUMC) en de Universiteit Leiden werken samen aan het vermeerderen van…

Promotors: W. Jiskoot; G.J. Witkamp, Co-promotor: M.R. Nejadnik

The present thesis focuses on the pharmacological concept of drug-target interaction, which dates back to the beginning of modern pharmacology.

The work described in this dissertation contributes to a better mechanistic understanding of nanoparticles in vivo. To achieve that goal, we used the zebrafish as a highly predictive pre-screening model of nanoparticles.

This joint Master with the Autonomous University of Barcelona will the equip you with the necessary competences to play a key role in the emerging field of transfusion medicine. The programme includes both transfusion of blood components and hematopoietic transplants, cellular and tissue therapy, and…

De GMP-faciliteit maaktde ontwikkeling en productie van Advanced Therapy Medicinal Products (ATMP's) en chemische (peptide) synthese mogelijk.

This doctoral thesis is an effort to understand how lipid phase-separation induced by diacylglycerol analogues in lipid-based nanoparticles affects their in vivo behavior, leading to specific nanoparticle-protein communications and selective cell targeting.

Despite being the second largest transmembrane protein family after GPCRs, the SLC superfamily has only gained significant attention in early drug development over the past decade.

Artsen moeten samen met patiënten met bot- en wekedelentumoren op zoek naar een optimale persoonlijke behandelstrategie, vindt prof. dr. Michiel van de Sande. Hij heeft een app ontwikkeld die bij de behandelkeuze ondersteunt. Van de Sande is vanaf 1 mei 2020 benoemd tot Leidse hoogleraar Orthopedie,…

Patiënten met baarmoederhalskanker hebben een grotere overlevingskans door een therapeutisch HPV-16 vaccin. Een nieuwe studie van het Leidse biotechnologie bedrijf ISA Pharmaceuticals B.V. en het Leids Universitair Medisch Centrum (LUMC) bewijst dat de response van het vaccin versterkt kan worden in…

This thesis examines silicon pore optics (SPO), a technology that exploits silicon wafers from the semiconductor industry to create extremely high quality X-ray optics, by studying its manufacturing process, applications, and prospects.

Clinical development of drugs for central nervous system (CNS) disorders has been particularly challenging and still suffers from high attrition rates.

Global healthcare is on the verge of an antibiotic availability crisis as bacteria have evolved resistance to nearly all known antibacterials. Identifying new antibiotics that operate via novel modes-of-action is therefore of high priority.

This dissertation explores new chemical strategies for the synthesis of self-adjuvanting peptide conjugates and immunomodulatory ligands aimed at targeted activation of the immune system.

Acute cardiovascular syndromes, including myocardial infarction or stroke, are the principal cause of death in the Western society. The main underlying pathology of cardiovascular diseases is atherosclerosis, which is caused by the accumulation of lipids and inflammatory cells in the vessel wall, in…

Promotor: F.S. de Boer Co-promotor: P. T. de Gouw

Tracing the evolution of the molecular gas content in galaxies is critical for a complete understanding of galaxy formation and evolution, as it provides the direct fuel for star formation. Studies of high-redshift (z>1) molecular gas reservoirs, most commonly traced by carbon monoxide (CO), have seen…

Promotores: B. van de Water; P. Hogendoorn; J. Bovée Co-Promotor: E.H.J. Danen

Promotor: Prof.dr. A. P. IJzerman, Co-Promotor: L.H. Heitman

As leading cause of death worldwide, cancer is responsible for nearly 10 million deaths in 2020 according to World Health Organization (WHO). Cisplatin and its derivatives are commonly used chemotherapy agents for current cancer treatment in the clinics.

Breast cancer is the most common cancer in women worldwide. Metastasis, the process by which cancer cells spread to other parts of the body, makes treatments less effective. It is important to unravel the mechanism of metastasis.

In this thesis, the focus lies on studying glucocorticoid receptor dynamics in living cells with the aim of understanding how this transcription factor finds its DNA target sites to regulate transcription.

Het eigen afweersysteem van een kankerpatiënt versterken zodat het lichaam zelf beter kankercellen kan vernietigen. Het is de kern van immuuntherapie, de hoopgevendste vooruitgang binnen de behandeling van kanker van de afgelopen jaren. Om deze ontwikkeling verder aan te jagen slaan het Antoni van Leeuwenhoek/Nederlands…

The aim of this thesis was to study phage infection in Streptomyces, focused on cell wall-deficient cells. Bacteriophages (or phages in short) attach to the cell wall of bacteria after which they replicate and lyse the host cell.

Promotor: M.A.G.J. Orrit

This thesis explores squaramide-based supramolecular polymers and hydrogels, enhancing their mechanical strength for 3D cell culture.

Microglia support the central and peripheral nervous system by forming the first line of defense against hazardous events. Microglia are increasingly acknowledged as critical players in neurodegenerative diseases, highlighting them as potential therapeutic targets.

Promotor: A.P. IJzerman Co-promotor: L.H. Heitman

Promotores: Prof.dr. H.S. Overkleeft, Prof.dr. G.A. van der Marel

The main topic of this PhD thesis is the Arakelov ray class group of a number field, an algebraic object that contains both the ideal class group structure and the unit group structure.

Gold nanorods are biocompatible nanoparticles that present an excellent two-photon signal that can be used to get high spatial resolution inside living cells. Gold nanorods are photostable and therefore can be followed inside cells for long time, with possible applications as trackers in live cells.

Prof. dr. Pancras Hogendoorn CW (LUMC), dr. Annemarie H. Meijer (IBL), prof. dr. Thomas Schmidt (LION) en dr. B. Ewa Snaar-Jagalska (IBL) kregen een interdisciplinaire NWO TOP subsidie voor hun project “Role of Chemokine Gradient Sensing in Ewing’s Sarcoma Progression, Angiogenesis and Immune Target…

Internist- klinisch farmacoloog Teun van Gelder is per 1 december 2019 benoemd tot Leidse hoogleraar Drug Discovery & Development. Hij zal zich bezighouden met het ontwikkelen van geneesmiddelen binnen de academische wereld, ook wel Academic Pharma genoemd.

Cell-based medicinal products (CBMPs), such as CAR T cells, are highly complex biopharmaceutical products offering various treatment options for serious illnesses such as cancer or autoimmune diseases.

Cardiovascular disease is a major global burden and atherosclerosis is the main underlying pathological process.

This thesis describes biochemical investigations of glucocerebrosidase (GBA), the lysosomal β- glucosidase that is deficient in Gaucher disease (GD).

Promotor: R.C.M. Pelger Co-Promotor: G. van der Pluijm

Promotor: W. Jiskoot, Co-promotor: A. Hawe

Prostate cancer (PCa) is one of the most prevalent cancer in males.

Microscopic insight on lipid-based nanomedicine in vivo remains limited to the perception of the knowledge that could be obtained: if we interpret only what we see, then we only believe to know. Although believing to know encapsulates an undefined amount of uncertainty in the exploration of lipid-based…

This thesis advances our understanding of lipid uptake, a vital first step in lipid metabolism, by developing innovative click chemistry-based tools to study how immune cells internalize lipids.

Control of infectious diseases poses continuous challenges for human health.

Phosphorylation affects all four major biomolecules – proteins, lipids, carbohydrates and nucleic acids – and plays a pivotal role in the most fundamental cellular functions.