This thesis comprises the development of reproducible synthesis procedures for two-dimensional materials (composed of boron, nitrogen and carbon) on different metallic surfaces.

The aim of this thesis is to develop and study a robust and adaptable scaffold for supramolecular polymer self-assembly in water.

Nanoparticles can be used as delivery systems for both small molecules and macromolecules such as proteins, peptides or oligonucleotides. This thesis focuses on the use of liposomes, nanometric vesicles formed by a lipid bilayer enclosing an aqueous core. Liposomes are highly versatile delivery syst…

Promotie

The emergence of complex diseases resulting from abnormal cell-cell signaling and the spread of infectious diseases caused by pathogens are significant threats to humanity. Unraveling the dynamic mechanisms underlying cell-cell signaling and infectious disease spreading is crucial for effective disease…

This thesis examines enzyme domain architecture and engineering to improve the breakdown of lignocellulose and starch, abundant biopolymers crucial for sustainable biofuel and biochemical production. Carbohydrate-active enzymes (CAZymes) facilitate this degradation, but the structural complexity of…

Drug induced organ toxicity is the main problem of the drug development and drug usage in the clinic. The liver and kidneys are the most sensitive organs towards drug induced toxicity.

How can we, together international experts, define future needs of R+D contributions for innovations in the field of risk-based management of chemicals and products in a global perspective using alternative testing strategies to minimize animal tests?

What are the behaviours of plant traits throughout various points in the growing season in a radiative transfer model framework and how well can this knowledge be integrated through data assimilation to provide priors for robust local and global vegetation products and analysis?

In this thesis population pharmacokinetic and physiologically-based pharmacokinetic (PBPK) approaches were applied to investigate the influence of glomerular filtration (GF) and active tubular secretion (ATS) on renal clearance in children.

Exploring metabolomics-based biomarkers for diagnosis and treatment response monitoring of community-acquired pneumonia

Barrie Sander identificeert verschillende risico’s en zorgen rondom het gebruik van kunstmatige intelligentie om klimaatverandering aan te pakken.

We found that, next to the effectiveness of the intervention, it’s also very likely that the intervention is cost-effective compared to attention only.

Hoe kan de methode om de gecombineerde effecten van metaal-gebaseerde chemische stoffetn te bepalen worden verbeterd?

This thesis aimed to investigate core cross-linked polymeric micelles (CCPMs) and expand their potential for the delivery of hydrophobic drugs and co-factors.

Climate change is a challenge for both current and future generations. New biological resources have to be developed in order to meet the demand for energy as well as the demand for food.

Docenten spelen een cruciale rol bij het implementeren van onderwijsvernieuwingen. Uiteindelijk hangt het succes van de implementatie af van de manier waarop docenten de vernieuwing vormgeven in hun dagelijkse lespraktijk.

In dit artikel onderzoekt Rutger Leukfeldt, bijzonder hoogleraar Governing Cybercrime, met collega's de relatie tussen PMT-factoren en zelfbeschermend onlinegedrag.

De huidige en toekomstige quantumcomputers vormen dezelfde uitdaging als de laser in zijn begindagen. In theorie werd voorspeld dat de laser een bron van zeer speciaal, zeer krachtig licht zou zijn. Maar in die tijd waren er geen duidelijke toepassingen voor. Critici van het idee noemden het een probleem…

Glycoside hydrolases (glycosidases/GHs) are widely abundant enzymes in all kingdoms of life and are important biocatalysts that catalyze the hydrolysis of glycosidic linkages in oligo/polysaccharides, glycoproteins and glycolipids with tremendous efficiency

The research described in this thesis centered on retaining exo-β-glucosidases implicated in health and disease, in particular the human inherited lysosomal storage disorder, Gaucher disease (GD).

Promotores: Prof.dr. H.S. Overkleeft, Prof.dr. G.A. van der Marel

Promotor: H.S. Overkleeft, Co-promotor: M. van der Stelt

Promotor: Prof.dr. M. Ubbink, Co-promotor: G. Siegal

Pseudomonas Aeruginosa is a Gram-negative bacterium which can form biofilms, increasing its resistance against antibiotics and the host immune system. Polysaccharides are an integral part of this biofilm, one of these polysaccharides is called Psl. PslG is a glycosidase, able to cleave this polysaccharide,…

Promotors: Prof.dr. E. Bouwman, Prof.dr. E. Drent

To this day, all cyclophellitol-based inhibitors and ABPs have been close analogues of their natural substrate counterparts. As a result, these probes showed high selectivity towards their target glycosidases.

This thesis describes the development and optimization of the first molecular tools to study the enzyme PLA2G4E.

What are trait-trait relationships in wetlands? To what extent the so-called leaf economics traits deviate from terrestrial ecosystems? How traits specific to wetland plant relate to plant strategies? What’s the relationship between wetland plant traits to the ecosystem functioning (e.g. productivity,…

This thesis describes the synthesis and biochemical evaluation of a variety of cyclophellitol based activity-based probes and inhibitors targeting various endo- and exo-acting retaining glycosidases. In the last two decades a variety of probes and inhibitors for (hemi)cellulose degrading enzymes have…

As leading cause of death worldwide, cancer is responsible for nearly 10 million deaths in 2020 according to World Health Organization (WHO). Cisplatin and its derivatives are commonly used chemotherapy agents for current cancer treatment in the clinics.

The chemical synthesis of inhibitors and probes targeting endo-glycosidases.

Vitamin A or retinol is essential in embryonic development, the visual cycle and the immune system.

How to make a quantitative assessment of the relative contribution to toxicity of ions and particles? Is the toxicity of nanoparticles related to their size or shape? Which physicochemical property of nanoparticles is an appropriate dose metric of nanoparticles?

Promotor: Prof.dr. G.A. van der Marel, Co-Promotor: J.D.C. Codée

Didactiek

Atoms and molecules are the basic units of matter. If we keep dividing a bar of gold or a glass of water into smaller parts, at the end we are left with a single gold atom or a water molecule.

During embryonic growth, cells proliferate, differentiate, and collectively migrate to form different tissues at the right position and time in the body.

This thesis focuses on the design and synthesis of activity-based probes and inhibitors targeting enzymes involved in the degradation of specific plant glycans.

This thesis describes the importance of being able to control the selectivity of potential drug candidates.

Promotor: Prof.dr. M. Ubbink

Promotor: Prof.dr. R.M.H. Merks

Which traits and/or trade-offs determine benefits of being deciduous or evergreen?

Large parts of the research described in this Thesis aims at the development of oligopeptide-masked toxins and their in situ immunoproteasome-mediated activation.

This thesis describes the synthesis, analysis of various tetrazine bearing compounds and use in bioorthogonal chemistry.

In this thesis an activity-based probe was discovered that could visualize the activity of PLAATs. With an optimized gel-based ABPP assay in hand, screening of a compound library led to the discovery of alpha-ketoamides as a hit for PLAAT3.

Promotor: J. van der Greef; Co-promotor: E. van Wijk, M. Wang

Promotores: Prof.dr. T.H.W. Bäck, Prof.dr. M.R.V. Chaudron, Co-Promotor: M.T.M. Emmerich